Animal product free media and processes for obtaining abotulinumtoxin

  • US 7,148,041 B2
  • Filed: 09/25/2003
  • Issued: 12/12/2006
  • Est. Priority Date: 09/25/2003
  • Status: Active Grant
First Claim
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1. A method for obtaining a biologically active botulinum toxin, comprising the steps of:

  • (a) providing a fermentation medium which is free of an animal product;

    (b) culturing a Clostridium botulinum bacterium in the fermentation medium under conditions which permit production of a botulinum toxin, and;

    (c) recovering a biologically active botulinum toxin from the fermentation medium,wherein the fermentation medium comprises a protein product obtained from yeast or from a vegetable, and wherein the vegetable is selected from the group consisting of soy, malt and corn.

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