Method for measuring substance and testing piece

• US 7,153,696 B2
• Filed: 03/11/2003
• Issued: 12/26/2006
• Est. Priority Date: 10/30/1995
• Status: Expired due to Fees

• Current Assignee: ARKRAY, Inc
• Original Assignee: ARKRAY, Inc
• Inventors: Yamamoto, Kenji, Yonehara, Satoshi, Fukuoka, Takao, Katayama, Atsuko
• Primary Examiner(s): Cole, Monique T.
• Application Number:
  US10/384,605
• Publication Number:
  US 20030166295A1
• Time in Patent Office: 1,386 Days
• Field of Search: 422/56, 422/57, 436/164, 436/169, 436/178
• US Class Current: 436/164
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: G01N 21/272 for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 31/00 Investigating or analysing non-biological materials by the use of the chemical methods specified in the subgroup; Apparatus specially adapted for such methods
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
• CPC Class Codes: Y10T 436/23 Carbon containing
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A method of measuring an analyte, comprising a step of measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein a layered inorganic compound is caused to exist in the reaction system including the formation reaction of the detectable substance, whereby high-sensitivity measurement is made possible, the detectable substance can be stabilized to improve accuracy of the measurement, a rate of a chemical reaction is increased to enable quick measurement, and high-sensitivity measurement is made possible even in a reaction system which forms an insoluble substance. Also, it can be provided an analytical testing piece for measuring an analyte, by measuring a detectable substance by using a reaction system including a formation reaction of the detectable substance based on a chemical reaction of the analyte contained in a sample, wherein the testing piece comprises at least one test portion having a detection portion for detecting the detectable substance and contains a layered inorganic compound at least in the test portion, whereby diffusion and elution of a dyestuff or the like is prevented, more sensitive and accurate simple analysis is made possible, and easy handling is possible.

Claims

1. A method for measuring an analyte, comprising:

a step of forming a detectable substance, which is formed by a chemical reaction of the analyte and a reagent and is formed with a quantitative correlation with the analyte, wherein a reaction system which forms the detectable substance contains a substance which decomposes the detectable substance, and the reaction system which forms the detectable substance contains a layered inorganic compound to suppress decomposition of the detectable substance; and

a step of measuring the detectable substance by an electrochemical method,

wherein the detectable substance is an electron carrier.

2. A method for measuring an analyte, comprising:

a step of forming a detectable substance, which is formed by a chemical reaction of the analyte and a reagent and is formed with a quantitative correlation with the analyte, wherein a reaction system which forms the detectable substance contains a substance which decomposes the detectable substance, and the reaction system which forms the detectable substance contains a layered inorganic compound to suppress decomposition of the detectable substance; and

a step of measuring the detectable substance,

wherein the formation reaction of the detectable substance is an oxidation-reduction reaction and the reaction system including the formation reaction of the detectable substance includes a formation reaction of hydrogen peroxide or an oxidation reaction using hydrogen peroxide as an oxidizing agent.

3. The method according to claim 2, which measures the analyte by colorimetrically determining a quantity of a dyestuff having a quantitative correlation with the analyte and formed by an oxidation-reduction reaction between hydrogen peroxide formed from the analyte through a chemical reaction and an oxidizable color coupler, wherein at least decomposition of the dyestuff by hydrogen peroxide is suppressed by causing the layered inorganic compound to exist in a system of the oxidation-reduction reaction.

4. A method for measuring an analyte, comprising:

a step of forming a detectable substance, which is formed by a chemical reaction of the analyte and a reagent and is formed with a quantitative correlation with the analyte, wherein a reaction system which forms the detectable substance contains a substance which decomposes the detectable substance, and the reaction system which forms the detectable substance contains a layered inorganic compound to suppress decomposition of the detectable substance; and

a step of measuring the detectable substance,

wherein the formation reaction of the detectable substance is an oxidation-reduction reaction and the reaction system including the formation reaction of the detectable substance includes the formation reaction of nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate or a reaction in which nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate acts as a reducing agent.

5. A method for measuring an analyte, comprising:

a step of forming a detectable substance, which is formed by a chemical reaction of the analyte and a reagent and is formed with a quantitative correlation with the analyte, wherein a reaction system which forms the detectable substance contains a substance which decomposes the detectable substance, and the reaction system which forms the detectable substance contains a layered inorganic compound to suppress decomposition of the detectable substance; and

a step of measuring the detectable substance,

wherein the detectable substance is an azo compound detected by an optical method, and the method measures the analyte by determining a quantity of an azo compound having a quantitative correlation with the analyte and formed by a coupling reaction between a diazonium salt formed from the analyte through a chemical reaction and a coupling reagent, wherein decomposition of the azo compound is suppressed by causing the layered inorganic compound to exist in a system of the coupling reaction.