Peptides for enhanced cell attachment and growth

• US 7,157,275 B2
• Filed: 09/26/2003
• Issued: 01/02/2007
• Est. Priority Date: 08/15/2003
• Status: Active Grant

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Liebmann-Vinson, Andrea, Heidaran, Mohammad A., Chaney, Bryce Nelson, Guarino, Richard David
• Primary Examiner(s): Carlson; Karen Cochrane
• Assistant Examiner(s): TSAY, MARSHA M
• Application Number:
  US10/670,771
• Publication Number:
  US 20050106727A1
• Time in Patent Office: 1,194 Days
• Field of Search: 525/450, 525/54.1, 530/324, 530/330, 530/345, 530/402, 435/402, 435/325, 435/368, 435/370, 435/404
• US Class Current: 435/325
• CPC Class Codes: B33Y 80/00 Products made by additive manufacturing
• CPC Class Codes: C07K 5/0806 the side chain containing 0 or 1 carbon atoms, i.e. Gly, Ala
• CPC Class Codes: C07K 5/1008 the side chain containing 0 or 1 carbon atoms, i.e. Gly, Ala
• CPC Class Codes: C07K 5/1019 with the first amino acid being basic
• CPC Class Codes: C07K 7/06 having 5 to 11 amino acids

Abstract

The present invention relates to cell adhesion promoting (“CAP”) peptide combinations that promote cell attachment or cell adhesion to culture surfaces that are otherwise cell adhesion resistant “CAR”. The invention provides combination of peptides that, when covalently coupled to a CAR layer such as hyaluronic acid that has been created on a polystyrene surface, promote cell attachment, growth differentiation, and execution of other desired cellular functions in culture.

Claims

1. A method for growing adherent cells, comprising:

(a) providing a cell adhesion promoting (CAP) peptide-modified polymer composition, said composition comprising a polymer surface, a hyaluronic acid (HA) layer bonded to said polymer surface, and one or more pentapeptides covalently bound to the HA layer, wherein said pentapeptides promote adherence of cells that substantially do not adhere to the HA layer in the absence of said pentapeptides;

(b) adding adherent cells in a growth-promoting culture medium to said composition;

(c) allowing said adherent cells to adhere to said composition; and

(d) incubating said adherent cells in the culture medium for a selected period of time under conditions that support cell growth;

thereby growing said adherent cells.

2. The method of claim 1 wherein said cells are fibroblasts or osteoblasts.

3. The method of claim 1 wherein said polymer surface is formed from a flexible material.

4. The method of claim 1 wherein said cells are liver cells.

5. The method of claim 1 wherein said cells are human or rat primary hepatocytes or porcine hepatocytes.

6. The method of claim 1 wherein said one or more pentapeptides lack an RGD sequence.

7. The method of claim 1, further comprising removing unattached (nonadherent) cells from the culture medium.

8. A method for growing adherent liver cells, comprising:

(a) providing a cell adhesion promoting (CAP) peptide-modified polymer composition, said composition comprising a polymer surface, a cell adhesion resistant (CAR) material bonded to said polymer surface, and one or more pentapeptides covalently bound to the CAR material, wherein said pentapeptides promote adherence of liver cells that substantially do not adhere to the CAR material in the absence of said pentapeptides;

(b) adding adherent liver cells in a growth-promoting culture medium to said composition;

(c) allowing said liver cells to adhere to said composition; and

(d) incubating said adherent liver cells in the culture medium for a selected period of time under conditions that support liver cell growth;

thereby growing said adherent liver cells.

9. The method of claim 8 wherein said cell adhesion resistant (CAR) material is hyaluronic acid (HA), alginic acid (AA), polyethylene glycol (PEG), or polyhydroxyethyl methacrylate (poly-HEMA).

10. The method of claim 9 wherein said cell adhesion resistant (CAR) material is hyaluronic acid (HA).

11. The method of claim 8 wherein said liver cells are human or rat primary hepatocytes or porcine hepatocytes.

12. The method of claim 8 wherein said one or more pentapeptides lack an RGD sequence.

13. The method of claim 8, further comprising removing unattached (nonadherent) liver cells from the culture medium.

14. A method for growing adherent liver cells, comprising:

(a) providing a cell adhesion promoting (CAP) peptide-modified polymer composition, said composition comprising a polymer surface, a hyaluronic acid (HA) layer bonded to said polymer surface, and one or more pentapeptides covalently bound to the HA layer, wherein said pentapeptides promote adherence of liver cells that substantially do not adhere to the HA layer in the absence of said pentapeptides;

(b) adding adherent liver cells in a growth-promoting culture medium to said composition;

(c) allowing said adherent liver cells to adhere to said composition; and

(d) incubating said adherent liver cells in the culture medium for a selected period of time under conditions that support liver cell growth;

thereby growing said adherent liver cells.

15. The method of claim 14 wherein said liver cells are human or rat primary hepatocytes or porcine hepatocytes.

16. The method of claim 14 wherein said one or more pentapeptides lack an RGD sequence.

17. The method of claim 14, further comprising removing unattached (nonadherent) liver cells from the culture medium.

18. A method for growing adherent cells, comprising:

(a) providing a cell adhesion promoting (CAP) peptide-modified polymer composition, said composition comprising a polymer surface, a hyaluronic acid (HA) layer bonded to said polymer surface, and one or more peptides covalently bound to the HA layer, wherein said peptides promote adherence of cells that substantially do not adhere to the HA layer in the absence of said peptides and wherein said one or more peptides lack an RGD sequence;

(b) adding adherent cells in a growth-promoting culture medium to said composition;

(c) allowing said adherent cells to adhere to said composition; and

(d) incubating said adherent cells in the culture medium for a selected period of time under conditions that support cell growth;

thereby growing said adherent cells.

19. The method of claim 18 wherein said cells are liver cells.

20. The method of claim 18, further comprising removing unattached (nonadherent) cells from the culture medium.

21. A method for growing adherent liver cells, comprising:

(a) providing a cell adhesion promoting (CAP) peptide-modified polymer composition, said composition comprising a polymer surface, a cell adhesion resistant (CAR) material bonded to said polymer surface, and one or more peptides covalently bound to the CAR material, wherein said peptides promote adherence of liver cells that substantially do not adhere to the CAR material in the absence of said peptides and wherein said one or more peptides lack an RGD sequence;

(b) adding adherent liver cells in a growth-promoting culture medium to said composition;

(c) allowing said liver cells to adhere to said composition; and

(d) incubating said adherent liver cells in the culture medium for a selected period of time under conditions that support liver cell growth;

thereby growing said adherent liver cells.

22. The method of claim 21 wherein said cell adhesion resistant (CAR) material is hyaluronic acid (HA), alginic acid (AA), polyethylene glycol (PEG), or polyhydroxyethyl methacrylate (poly-HEMA).

23. The method of claim 22 wherein said cell adhesion resistant (CAR) material is hyaluronic acid (HA).

24. The method of claim 21, further comprising removing unattached (nonadherent) liver cells from the culture medium.