Fluid handling apparatus for an automated analyzer

• US 7,182,912 B2
• Filed: 05/29/2002
• Issued: 02/27/2007
• Est. Priority Date: 03/04/1991
• Status: Expired due to Term

• Current Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: Bayer Corporation (Bayer AG)
• Inventors: Klingshirn, Frank C., Whitesel, Mary Beth, Carey, Glen, Lewis, Scott C.
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): Cross; LaToya I.
• Application Number:
  US10/156,849
• Publication Number:
  US 20030194349A1
• Time in Patent Office: 1,735 Days
• Field of Search: 422/64, 422/63, 422/65, 422/102, 436/43, 436/45, 436/42, 366/214, 366/218, 494 33- 34, 494/46, 494/19
• US Class Current: 422/64
• CPC Class Codes: B01L 2300/0854 Double walls
• CPC Class Codes: B01L 3/508 rigid containers not provided for above
• CPC Class Codes: G01N 2035/00386 using fluid heat transfer medium
• CPC Class Codes: G01N 2035/00396 where the fluid is a liquid
• CPC Class Codes: G01N 2035/00524 Mixing by agitating sample carrier
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 2035/0441 for samples
• CPC Class Codes: G01N 2035/0443 for reagents
• CPC Class Codes: G01N 2035/0465 Loading or unloading the conveyor
• CPC Class Codes: G01N 2035/0486 Gearing, cams
• CPC Class Codes: G01N 2035/1025 Fluid level sensing
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: G01N 35/021 having a flexible chain, e.g. "cartridge belt", conveyor for reaction cells or cuvettes
• CPC Class Codes: G01N 35/025 having a carousel or turntable for reaction cells or cuvettes
• CPC Class Codes: G01N 35/1002 Reagent dispensers
• CPC Class Codes: G01N 35/1065 Multiple transfer devices
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/111666 Utilizing a centrifuge or compartmented rotor
• CPC Class Codes: Y10T 436/113332 with conveyance of sample along a test line in a container or rack

Abstract

An analyzer for performing automated assay testing. The analyzer includes a storage and conveyor system for conveying cuvettes to an incubation or processing conveyor, a storage and selection system for test sample containers, a storage and selection system for reagent containers, sample and reagent aspirating and dispensing probes, a separation system for separating bound from unbound tracer or labeled reagent, a detection system and date collection/processing system. All of the subunits of the machine are controlled by a central processing unit to coordinate the activity of all of the subunits of the analyzer. The analyzer is specifically suited for performing heterogeneous binding assay protocols, particularly immunoassays.

Claims

1. A high throughput, high sensitivity, random access, automated magnetic particle chemiluminescence immunoassay analyzer having a reagent transport apparatus for transporting reagent containers to at least one aspiration position and for maintaining solid phase reagent magnetic microparticles in suspension in liquid, the reagent transport apparatus comprising:

a plurality of agitating units disposed in a first circular array concentric with a primary vertical axis of rotation and collectively rotatable about the primary vertical axis of rotation, each of the agitating units comprising

a reagent container holder rotatable about a respective secondary vertical axis of rotation, and

a first toothed wheel coupled to the respective reagent container holder, the first toothed wheel being coaxial with the respective secondary vertical axis of rotation, whereby rotation of the first toothed wheel causes rotation of the respective reagent container holder about the respective secondary vertical axis of rotation;

a plurality of solid phase reagent containers, each containing a solid phase reagent comprised of paramagnetic microparticles in a liquid, each disposed upon a respective one of the reagent container holders of the agitating units, and each having an internal agitating feature formed therein for agitating the respective solid phase reagent;

a first motor for selectively rotating the plurality of agitating units about the primary vertical axis of rotation whereby a selected first reagent container is positionable at the at least one aspiration position;

a plurality of mounting units disposed in a second circular array concentric with the primary vertical axis of rotation and collectively rotatable about the primary vertical axis of rotation;

a plurality of labeled reagent containers, each containing chemiluminescence labeled reagent comprised of a chemiluminescence label and each disposed upon a respective one of the mounting units;

a reversible second motor having an output shaft extending therefrom; and

a second toothed wheel coupled to the output shaft of the second motor and in simultaneous mechanical communication with each of the first toothed wheels of the plurality of agitating units, whereby reversible rotation of the output shaft and the second toothed wheel by the second motor causes the first toothed wheels and the respective reagent container holders coupled thereto to rotate about the respective secondary vertical axes of rotation in an oscillatory manner, thereby causing each of the solid phase reagent containers disposed in conjunction with a respective one of the agitating units to rotate in an oscillatory manner and causing the internal agitating feature to agitate the contents of the respective container to suspend the paramagnetic microparticles in the liquid.

2. The reagent transport apparatus of claim 1, further comprising a third toothed wheel in simultaneous mechanical communication with the first toothed wheels of the plurality of agitating units and the second toothed wheel.

3. The reagent transport apparatus of claim 2, wherein the third toothed wheel is a first ring gear.

4. The reagent transport apparatus of claim 3, wherein the first ring gear is concentric with the primary vertical axis of rotation.

5. The reagent transport apparatus of claim 3, wherein the first ring gear has teeth on its outer circumference.

6. The reagent transport apparatus of claim 3, wherein the second toothed wheel is comprised of a second ring gear.

7. The reagent transport apparatus of claim 6, wherein the second ring gear is concentric with the primary vertical axis of rotation.

8. The reagent transport apparatus of claim 6, wherein the second ring gear has upwardly facing teeth about its outer periphery.

9. The reagent transport apparatus of claim 1, wherein the second toothed wheel is comprised of a ring gear.

10. The reagent transport apparatus of claim 9, wherein the ring gear is concentric with the prinary vertical axis of rotation.

11. The reagent transport apparatus of claim 9, wherein the ring gear has upwardly facing teeth about its outer periphery.

12. The reagent transport apparatus of claim 1, wherein each first toothed wheel of the plurality of agitating units is a pinion gear.

13. The reagent transport apparatus of claim 1, wherein the first motor is further operative to rotate the plurality of mounting units about the primary vertical axis of rotation.

14. The reagent transport apparatus of claim 1, further comprising a tray mounted for rotation about the primary vertical axis of rotation and in driving engagement with the first motor, wherein each of the plurality of agitating units is disposed upon the tray.

15. The reagent transport apparatus of claim 14, wherein each of the plurality of mounting units is disposed upon the tray.

16. The reagent transport apparatus of claim 1, wherein the internal agitating feature formed within each solid phase reagent container is a pair of coplanar agitating fins.

17. A method of transporting reagent containers in a high throughput, high sensitivity, random access, automated magnetic particle chemiluininescence immunoassay analyzer, the method of transporting comprising:

providing a plurality of agitating units in a first circular array concentric with a primary vertical axis of rotation, the plurality of agitating units being collectively rotatable about the primary vertical axis of rotation, each of the agitating units comprising

a reagent container holder rotatable about a respective secondary vertical axis of rotation, and

a first toothed wheel coupled to the respective reagent container holder, the first toothed wheel being coaxial with the respective secondary vertical axis of rotation, whereby rotation of the first toothed wheel causes rotation of the respective reagent container holder about the respective secondary vertical axis of rotation;

disposing each of a plurality of solid phase reagent containers in conjunction with a respective one of the reagent container holders of the agitating units, each of the solid phase reagent containers containing a solid phase reagent comprising paramagnetic microparticles in a liquid and having an internal agitating feature formed therein for agitating the respective solid phase reagent;

rotating the plurality of agitating units about the primary vertical axis of rotation via selective operation of a first motor whereby a selected first reagent container is positionable at at least one aspiration position;

providing a plurality of mounting units in a second circular array concentric with the primary vertical axis of rotation, the plurality of mounting units being collectively rotatable about the primary vertical axis of rotation;

disposing each of a plurality of labeled reagent containers in conjunction with a respective one of the mounting units, each of the plurality of labeled reagent containers containing labeled reagent comprising a chemiluminescence label;

providing a second motor having an output shaft;

providing a second toothed wheel in communication with the output shaft of the second motor and in simultaneous mechanical communication with each of the first toothed wheels of the plurality of agitating units; and

reversibly rotating the output shaft and the second toothed wheel by the second motor, thereby causing the first toothed wheels and the respective reagent container holders coupled thereto to simultaneously rotate about the respective secondary vertical axes of rotation in an oscillatory manner, thereby causing each of the solid phase reagent containers disposed upon a respective one of the agitating units to rotate in an oscillatory manner, and thereby causing the internal agitating feature within each of the plurality of solid phase reagent containers to agitate the contents of the respective solid phase reagent container to suspend the paramagnetic microparticles in the liquid.

18. The method of claim 17, further comprising the step of providing a third toothed wheel in simultaneous mechanical communication with the first toothed wheels of the plurality of agitating units and the second toothed wheel.

19. The method of claim 18, wherein the third toothed wheel is provided as a first ring gear.

20. The method of claim 19, wherein the first ring gear is provided concentric with the primary vertical axis of rotation.

21. The method of claim 19, wherein the first ring gear is provided having teeth on its outer circumference.

22. The method of claim 19, wherein the second toothed wheel is provided as comprising a second ring gear.

23. The method of claim 22, wherein the second ring gear is provided concentric with the primary vertical axis of rotation.

24. The method of claim 22, wherein the second ring gear is provided having upwardly facing teeth about its outer periphery.

25. The method of claim 17, wherein the second toothed wheel is provided as comprising a ring gear.

26. The method of claim 25, wherein the ring gear is provided concentric with the primary vertical axis of rotation.

27. The method of claim 25, wherein the ring gear is provided having upwardly facing teeth about its outer periphery.

28. The method of claim 17, wherein each first toothed wheel of the plurality of agitating units is provided as a pinion gear.

29. The method of claim 17, further comprising the step of rotating the plurality of mounting units about the primary vertical axis of rotation via selective operation of the first motor.

30. The method of claim 17, further comprising the steps of:

providing a tray mounted for rotation about the primary vertical axis of rotation and in driving engagement with the first motor; and

disposing each of the plurality of agitating units upon the tray.

31. The method of claim 30, further comprising the step of disposing each of the plurality of mounting units upon the tray.

32. The method of claim 17, wherein the step of disposing each of a plurality of solid phase reagent containers comprises randomly disposing each of the plurality of solid phase reagent containers in conjunction with a respective one of the reagent container holders of the agitating units.