Methods for analyzing polynucleotide targets using tethered oligonucleotide probes

  • US 7,192,707 B2
  • Filed: 11/06/2003
  • Issued: 03/20/2007
  • Est. Priority Date: 04/07/1995
  • Status: Expired due to Term
First Claim
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1. A method of analysis which comprises:

  • providing a polynucleotide target including a nucleotide at a specified position, and two or more different oligonucleotide probes tethered to different locations of a support in the form of an array, each said probe being complementary to the target including an expected variant of the target and terminating at or close to the said specified position; and

    performing the steps;

    (a) incubating the target with the probes to form duplex(es),(b) incubating the duplex(es) under (i) ligation conditions with a labelled oligonucleotide complementary to the target or (ii) chain extension conditions with at least one labelled nucleotide; and

    (c) monitoring addition of the nucleotide/oligonucleotide to a probe by ligation or chain extension in step (b) as an indication of a point mutation at the specified position in the target.

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