Fixed mounted sorting cuvette with user replaceable nozzle

• US 7,201,875 B2
• Filed: 09/27/2002
• Issued: 04/10/2007
• Est. Priority Date: 09/27/2002
• Status: Active Grant

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Javadi, Shervin, Norton, Pierce O., Vrane, David R.
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): Handy; Dwayne K
• Application Number:
  US10/259,332
• Publication Number:
  US 20040062685A1
• Time in Patent Office: 1,656 Days
• Field of Search: 422/68.1, 422/73, 422/82.05, 422/82.08, 422/82.09, 422/99, 422/100, 422/102, 356/335, 356/336, 356/337, 356/338, 356/441, 356/442
• US Class Current: 422/73
• CPC Class Codes: G01N 15/14 Optical investigation techniques, e.g. flow cytometry
• CPC Class Codes: G01N 15/1404 Handling flow, e.g. hydrodynamic focusing
• CPC Class Codes: Y10T 436/101666 Particle count or volume standard or control [e.g., platelet count standards, etc.]
• CPC Class Codes: Y10T 436/117497 with a continuously flowing sample or carrier stream
• CPC Class Codes: Y10T 436/119163 with aspirator of claimed structure

Abstract

A flow cell and flow cytometer in which a nozzle at the end of a flow channel is disposed on a removable substrate held at a registered location on a flow cell. Other elements including illumination optics, light collection optics, and the flow cell may then be positioned at fixed locations and would not require subsequent periodic adjustment. The registered location for positioning the nozzle allows removal and replacement of the nozzle key with the nozzle subsequently positioned in the identical location.

Claims

1. A flow cell for use with a flow cytometer comprising:

a flow cell body;

a sample delivery tube extending into said flow cell body;

at least one sheath flow port on said flow cell body, said at least one port allowing introduction of a flow of sheath flow liquid through said flow cell body;

a cuvette having flat sides and a rectanaular cross-section, joined to the flow cell body;

a channel extending through the cuvette, said channel comprising an initial end and a terminal end, wherein liquid from said sample delivery tube and said at least one sheath flow port flows into said initial end of said channel; and

a removable nozzle at said terminal end of said channel through which said liquid from said sample delivery tube and said at least one sheath flow port flows out of said channel, wherein said removable nozzle is positioned on a removable nozzle key having hard planar surfaces on a top and on at least two sides, said nozzle held in a registered position on said flow cell, said registered position at a defined three-dimensional position and at a registered rotational orientation, wherein said nozzle is held in said registered position by contact between said hard planar surfaces of said nozzle key and said cuvette.

2. The flow cell of claim 1, further including a droplet generator associated with said flow cell, said droplet generator allowing production of droplets as a stream exits the nozzle.

3. The flow cell of claim 2, wherein said droplet generator is located within said flow cell body such that droplet generation oscillations are imparted to flow fluid before said fluid enters the channel.

4. The flow cell of claim 3, wherein said droplet generator includes a piston.

5. The flow cell of claim 2, wherein said droplet generator is a vibrating element.

6. The flow cell of claim 5, wherein said vibrating element vibrates one of a group consisting of the nozzle, the cuvette, and the flow cell body.

7. The flow cell of claim 1, wherein said nozzle key includes a biasing spring on one side of said key.

8. The flow cell of claim 1, wherein said nozzle key includes an o-ring surrounding the nozzle.

9. The flow cell of claim 1, wherein said cuvette has optically transmissive sidewalls that extend downward from a location of said terminus of said channel.

10. The flow cell of claim 1, wherein said channel baa a rectangular cross section.

11. The flow cell of claim 1, wherein said channel has a length sufficiently long so that liquid flowing through said channel has fully developed flow when said fluid reaches an illumination location on said channel.

12. The flow cell of claim 1, wherein when said nozzle is inserted into said registered location, a nozzle opening of to nozzle is off center from a centered position within the channel.

13. A flow cytometer system comprising:

(a) a flow cell, wherein said flow cell comprises

a flow cell body;

a sample delivery tube extending into said flow cell body;

at least one sheath flow port on said flow cell body, said at least one port allowing introduction of a flow of sheath flow liquid through said flow cell body;

a cuvette having flat sides and a rectangular cross-section, joined to the flow cell body;

a channel extending through the cuvette, said channel comprising an initial end and a terminal end, said channel joined to said flow cell body such tat liquid from said sample delivery tube and said at least one sheath flow port flows into said initial end of said channel; and

a removable nozzle at said terminal end of said channel through which said liquid from said sample delivery tube and said at least one sheath flow port flows out of said channel, wherein said removable nozzle is positioned on a removable nozzle key having hard planar surfaces on a top and on at least two sides said nozzle held in a registered position on said flow cell, said registered position at a defined three-dimensional position and at a registered rotational orientation, wherein said nozzle is held in said registered position by contact between said hard planar surfaces of said nozzle key and said cuvette;

(b) illumination optics which focus and direct illumination light into said flow cell at an illumination region of said flow cell; and

(c) light collection optics which collect light produced from targets in the channel of the flow cell and transmit collected light to detection optics.

14. The system of claim 13, further including a droplet generator associated with said flow cell, said droplet generator allowing production of droplets as a stream exits the nozzle.

15. The system of claim 14, wherein said droplet generator is located within said flow cell body such that droplet generation oscillations are imparted to flow fluid before said fluid enters the channel.

16. The system of claim 15, wherein said droplet generator includes a piston.

17. The system of claim 14, wherein said droplet generator is a vibrating element.

18. The system of claim 17, wherein said vibrating element vibrates one of a group consisting of the nozzle, the cuvette, and the flow cell body.

19. The system of claim 13, wherein said nozzle key includes a biasing spring on one side of said key.

20. The system of claim 13, wherein said nozzle key includes en o-ring surrounding the nozzle.

21. The system of claim 13, wherein said cuvette has optically transmissive sidewalls that extend downward from a location of said terminus of said channel.

22. The system of claim 13, wherein said channel has a rectangular cross section.

23. The system of claim 13, wherein said channel has a length sufficiently long so that liquid flowing through said channel has fully developed flow when said fluid reaches the illumination region.

24. The system of claim 13, wherein when said nozzle is inserted into said registered location, a nozzle opening of the nozzle is off center from a centered position within the channel.

25. The system of claim 13, further including alight transmissive coupling joining the cuvette with a light collection lens, said lens being an element of the light collection optics.

26. The system of claim 22, wherein said channel is rectangular and has a shorter cross-sectional side and a longer cross-sectional side, wherein said shorter cross-sectional side faces a first direction in which light is directed by the illumination optics and the longer cross-sectional side faces a second direction from which emitted light is collected by the light collection optics.