Δ-12 desaturase gene suitable for altering levels of polyunsaturated fatty acids in oleaginous yeasts
First Claim
Patent Images
1. An isolated nucleic acid molecule encoding a Yarrowia Δ
- 12 desaturase enzyme, selected from the group consisting of;
(a) an isolated nucleic acid molecule encoding the amino acid sequence as set forth in SEQ ID NO;
24;
(b) an isolated nucleic acid molecule that hybridizes with (a) under the following hybridization conditions;
0.1X SSC, 0.1% SDS, 65°
C. and washed with 2X SSC, 0.1% SDS followed by 0.1X SSC, 0.1% SDS;
oran isolated nucleic acid molecule that is complementary to (a) or (b).
3 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to a Δ12 fatty acid desaturase able to catalyze the conversion of oleic acid to linoleic acid (LA; 18:2). Nucleic acid sequences encoding the desaturase, nucleic acid sequences that hybridize thereto, DNA constructs comprising the desaturase gene, and recombinant host microorganisms expressing increased levels of the desaturase are described. Methods of increasing production of specific ω-3 and/or ω-6 fatty acids are described by overexpression of the Δ12 fatty acid desaturase or by disruption of the native gene.
46 Citations
10 Claims
-
1. An isolated nucleic acid molecule encoding a Yarrowia Δ
- 12 desaturase enzyme, selected from the group consisting of;
(a) an isolated nucleic acid molecule encoding the amino acid sequence as set forth in SEQ ID NO;
24;(b) an isolated nucleic acid molecule that hybridizes with (a) under the following hybridization conditions;
0.1X SSC, 0.1% SDS, 65°
C. and washed with 2X SSC, 0.1% SDS followed by 0.1X SSC, 0.1% SDS;
oran isolated nucleic acid molecule that is complementary to (a) or (b). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
- 12 desaturase enzyme, selected from the group consisting of;
-
9. A method for modulating the biosynthesis of ω
- -3 or ω
-6 fatty acids in a Yarrowia host cell comprising;a) providing a Yarrowia host cell comprising a functional ω
-3/ω
-6 fatty acid biosynthetic pathway;b) over-expressing a Δ
12 desaturase gene encoding the Δ
12 desaturase enzyme as set forth in SEQ ID NO;
24 in the host cell of (a);
whereby the biosynthesis of ω
-3 or ω
-6 fatty acids is modulated.
- -3 or ω
-
10. A method of obtaining a nucleic acid molecule encoding a Δ
- 12 desaturase enzyme comprising;
(a) synthesizing at least one oligonucleotide primer corresponding to a portion of the sequence as set forth in SEQ ID NOs;
23; and(b) amplifying an insert present in a cloning vector using the oligonucleotide primer of step (a); wherein the amplified insert encodes a portion of an amino acid sequence encoding a Δ
12 desaturase enzyme.
- 12 desaturase enzyme comprising;
Specification