Method of manufacturing electrochemical sensors

  • US 7,225,535 B2
  • Filed: 09/12/2003
  • Issued: 06/05/2007
  • Est. Priority Date: 10/08/1998
  • Status: Expired due to Term
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1. A method of manufacturing electrochemical sensors, the method comprising steps of:

  • (a) applying a plurality of working electrodes on a substrate;

    (b) applying a plurality of counter electrodes on the substrate;

    (c) positioning a spacer layer over the substrate and the working electrodes and the counter electrodes;

    (d) overlaying the spacer layer with a second substrate;

    (e) creating a sample chamber region within the spacer layer comprising a plurality of connected sample chambers between the substrate and the second substrate; and

    (f) separating a plurality of electrochemical sensors, each electrochemical sensor comprising at least one working electrode planar with at least one counter electrode on the substrate, the substrate and second substrate having generally a similar length and width, and at least one sample chamber between the substrate and the second substrate, the sample chamber having a volume of no more than about 1 microliter and continuous with the at least one sample chamber of an adjacent electrochemical sensor.

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