Specific binding agents for KSHV vIL-6 that neutralize a biological activity
First Claim
1. A method for monitoring a response to a treatment in a subject with a Kaposi'"'"'s sarcoma-associated herpesvirus (KSHV) associated disorder, comprisingcontacting a sample from the subject with a monoclonal antibody that specifically binds Kaposi'"'"'s sarcoma-associated herpesvirus (KSHV) interleukin-6 (vIL-6), wherein the monoclonal is produced by a hybridoma deposited as American Type Culture Collection (ATCC) Deposit No. PTA-2217, PTA-2218, PTA-2219, or PTA-2220 or is a humanized form thereof,detecting the binding of the monoclonal antibody with the sample,wherein binding of the monoclonal antibody to the sample is indicative of the response to the treatment of the subject with the KSHV associated disorder.
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Abstract
A specific binding agent is provided, wherein the specific binding agent specifically binds Kaposi'"'"'s sarcoma-associated herpesvirus (KSHV) interleukin-6 (vIL-6), and the specific binding agent neutralizes an activity of vIL-6. In one embodiment, the specific binding agent is an antibody. Methods are provided for using a specific binding agent that binds vIL-6, and neutralizes a biological activity of vIL-6. Methods of treatment for a KSHV-associated disorder are also provided. Methods for diagnosing a KSHV-associated disorder are provided, as are kits that include a specific binding agent of the invention. A method is also provided for testing an agent for effectiveness in treating a KSHV-associated disorder. The method includes incubating the agent with a cell free system comprising a vIL-6 receptor component and vIL-6, and comparing the binding of vIL-6 and the receptor component in the presence of the agent to binding of vIL-6 to the receptor component in the absence of the agent. A decrease in the binding of vIL-6 to the receptor component in the presence of the agent indicates that the agent is effective for treating the KSHV-associated disorder.
64 Citations
11 Claims
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1. A method for monitoring a response to a treatment in a subject with a Kaposi'"'"'s sarcoma-associated herpesvirus (KSHV) associated disorder, comprising
contacting a sample from the subject with a monoclonal antibody that specifically binds Kaposi'"'"'s sarcoma-associated herpesvirus (KSHV) interleukin-6 (vIL-6), wherein the monoclonal is produced by a hybridoma deposited as American Type Culture Collection (ATCC) Deposit No. PTA-2217, PTA-2218, PTA-2219, or PTA-2220 or is a humanized form thereof, detecting the binding of the monoclonal antibody with the sample, wherein binding of the monoclonal antibody to the sample is indicative of the response to the treatment of the subject with the KSHV associated disorder.
Specification