Method for rapid thermal cycling of biological samples

  • US 7,238,321 B2
  • Filed: 07/13/2004
  • Issued: 07/03/2007
  • Est. Priority Date: 06/04/1990
  • Status: Expired due to Fees
First Claim
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1. A thermal cycler for amplifying a nucleic acid comprising:

  • a housing comprising a chamber;

    a port in said housing for receiving at least one polymerase chain reaction (PCR) sample holder in said chamber;

    a heating assembly for heating a sample comprising a nucleic acid contained in said sample holder, wherein said heating assembly is in thermal communication with said PCR sample holder when said sample holder is present in said chamber;

    a cooling assembly for cooling said sample, wherein said cooling assembly is in thermal communication with said PCR sample holder when said PCR sample holder is present in said chamber; and

    a controller programmed to control said heating and said cooling assemblies to cycle said sample holder and said sample through a predetermined temperature cycle corresponding to the denaturation, annealing and elongation steps of a polymerase chain reaction within a time range of 30-60 seconds;

    wherein said PCR sample holder has a thermal mass which provides for completing said cycle of said polymerase chain reaction within said time range.

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