Methods of genetic analysis of mouse

• US 7,250,289 B2
• Filed: 11/20/2003
• Issued: 07/31/2007
• Est. Priority Date: 11/20/2002
• Status: Expired due to Fees

• Current Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Original Assignee: Affymetrix, Inc. (Thermo Fisher Scientific Incorporated)
• Inventors: Zhou, Xue Mei
• Primary Examiner(s): SITTON, JEHANNE SOUAYA
• Application Number:
  US10/719,900
• Publication Number:
  US 20050026164A1
• Time in Patent Office: 1,349 Days
• Field of Search: None
• US Class Current: 435/287.2
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C12Q 1/6888 for detection or identification of organisms
• CPC Class Codes: C12Q 2525/185 incorporating bases where the precise position of the bases in the nucleic acid string is important
• CPC Class Codes: C12Q 2545/101 with an internal standard/control
• CPC Class Codes: C12Q 2600/158 Expression markers

Abstract

The invention provides nucleic acid sequences which are complementary, in one embodiment, to a wide variety of mouse genes. The invention provides the sequences in such a way as to make them available for a variety of analyses. In one embodiment the nucleic acid sequences provided are present as an array of probes that may be used to measure gene expression of at least 30,000 mouse genes. As such, the invention relates to diverse fields impacted by the nature of molecular interaction, including chemistry, biology, medicine, and medical diagnostics.

Claims

1. An array comprising a plurality of nucleic acid probes, wherein the plurality comprises each of the sequences listed in SEQ ID NOS 1-982,914, and wherein each probe in the plurality of nucleic acid probes consists of one of the sequence listed in SEQ ID NOS:

1-982,914.

2. The array of claim 1 further comprising at least one probe that is the perfect complement of one of the sequences listed in SEQ ID Nos. 1-982,914.

3. The array of claim 1 further comprising at least one probe that is the mismatch probe corresponding to one of the sequences listed in SEQ ID Nos. 1-982,914, wherein the mismatch position is the central position.

4. The array of claim 1 wherein said plurality of nucleic acid probes is attached to a solid support.

5. The array of claim 1 wherein the array comprises a plurality of beads wherein the probes are attached to the beads and the probes on a bead consist of one of the sequences listed in SEQ ID Nos. 1-982,914.

6. The array of claim 1 wherein the array consists of a single contiguous solid support.