Light source wavelength correction

• US 7,254,503 B2
• Filed: 06/03/2004
• Issued: 08/07/2007
• Est. Priority Date: 06/03/2003
• Status: Active Grant

• Current Assignee: Siemens Healthcare Diagnostics Incorporated (Siemens AG)
• Original Assignee: Siemens Medical Solutions Diagnostics (Siemens AG)
• Inventors: Howard, Willis E. III
• Primary Examiner(s): Barlow; John
• Assistant Examiner(s): VO, HIEN XUAN
• Application Number:
  US10/560,440
• Publication Number:
  US 20060139649A1
• Time in Patent Office: 1,160 Days
• Field of Search: 702/85, 702/93, 702/28, 356319-320, 356/328, 356/445, 356/73.1, 356/39, 250/227.23, 250/226, 250/221, 250/205
• US Class Current: 702/85
• CPC Class Codes: B01L 2300/0825 Test strips
• CPC Class Codes: B01L 9/52 Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
• CPC Class Codes: G01J 2003/2866 Markers; Calibrating of scan
• CPC Class Codes: G01N 2021/0325 Cells for testing reactions, e.g. containing reagents
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/4738 Diffuse reflection precedence is given to G01N21/55 - G01N21/57 if specular component is taken into consideration, e.g. also for testing fluids, fibrous materials
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04
• CPC Class Codes: G01N 2201/0245 with insertable-removable part
• CPC Class Codes: G01N 2201/12792 Compensating own radiation in apparatus

Abstract

A wavelength correction function provides corrected reflectance values from actual reflectance values taken in a reflectance-base instrument. The correction is provided as a function of measured reflectance values and a predefined set of high resolution reflectance values established for the reflectance-based instrument implementing the wavelength correction function.

Claims

1. A method of correcting one or more reflectance values when a center wavelength of one or more light sources used to generate corresponding light signals is different from a specified center wavelength for the one or more light sources, the method comprising the steps of:

A. defining, for each of the one or more light sources, a reference spectral distribution {L*} that is characteristic of the one or more light sources and composed of reference light intensity values over a set of reference wavelengths;

B. defining, for each of the one or more light sources, a spectral distribution {L} comprising actual light intensity values over the set of wavelengths;

C. determining the actual reflectance R of a set of reflected signals;

D. defining a set of detector sensitivity data {D} corresponding to the set of detectors receiving the set of reflected signals;

E. determining high resolution reflectance values {r};

F. determining a correction factor as a function of {L}, {L*}, {r} and {D}; and

G. applying the correction factor to R to determine R*.

2. The method of claim 1, wherein determining the correction factor in step F is valid up to a range of at least about ±

8 nanometers around the specified center wavelength.

3. The method of claim 1, wherein the one or more light sources comprise LEDs.

4. The method of claim 1, wherein at least one of the one or more light sources is an infrared light source and determining {r} in step E comprises measuring reflectance values R_IR in the infrared range and determining r_IR as a constant representing an average of R_IR, where each value in {r} equals the value of (R/R_IR)·

r_IR at a corresponding wavelength.

5. The method of claim 4, wherein the values of {r} are determined at discrete wavelength intervals.

6. The method of claim 1, wherein the one or more light sources and set of detectors comprise part of a reflectometer.

7. The method of claim 1, wherein determining a correction factor as a function of {L}, {L*}, {r} and {D} comprises determining a correction factor

8. A center wavelength correction system configured to correct one or more reflectance values when a center wavelength of one or more light sources used to generate corresponding light signals is different from a specified center wavelength for the one or more light sources, the system comprising:

A. a spectral distribution module configured to determine, for each of the one or more light sources, a spectral distribution {L} comprising actual light intensity values over the set of wavelengths;

B. a reflectance module configure to determine actual reflectance R from a set of reflected signals;

C. at least one storage device comprising;

1) for each of the one or more light sources, a reference spectral distribution {L* } that is characteristic of the one or more light sources and composed of reference light intensity values over a set of reference wavelengths;

2) high resolution reflectance values {r}; and

3) detector sensitivity data {D} corresponding to the set of detectors receiving the set of reflected signals;

D. a correction function module configured to determine a correction factor at a given wavelength as a function of {L}, {L*}, {r} and {D} and to apply the correction factor to R to determine R*.

9. The system of claim 8, wherein the correction function module is configured to determine the correction factor within a range of at least about ±

8 nanometers around the specified center wavelength.

10. The system of claim 8, wherein the one or more light sources comprise LEDs.

11. The system of claim 8, wherein at least one of the one or more light sources is an infrared light source and the correction function is configured to determine {r} as a function of measured reflectance values R_IR an the infrared range and a constant r_IR that represents an average of R_IR, where each value in {r} equals the value of (R/R_IR)r_IR at a corresponding wavelength.

12. The system of claim 11, wherein the values of {r} are determined at discrete wavelength intervals.

13. The system of claim 8, wherein the one or more light sources and set of detectors comprise part of a reflectometer.

14. The system of claim 8, wherein the correction function module is configured to determine a correction factor

15. A wavelength correction means for correcting one or more reflectance values when a center wavelength of one or more light sources used to generate corresponding light signals is different from a specified center wavelength for the one or more light sources, the system comprising:

A. a spectral distribution means for determining, for each of the one or more light sources, a spectral distribution {L} comprising actual light intensity values over the set of wavelengths;

B. a reflectance means for determining actual reflectance R from a set of reflected signals;

C. at least one storage means far storing;

1) for each of the one or more light sources, a reference spectral distribution {L} that is characteristic of the one or more light sources and composed of reference light intensity values over a set of reference wavelengths;

2) high resolution reflectance values {r}; and

3) detector sensitivity data {D} corresponding to the set of detectors receiving the set of reflected signals;

D. a correction function means for determining a correction factor at a given wavelength as a function of {L}, {L*}, {r} and {D} and to apply the correction factor to R to determine R*.

16. The means of claim 15, wherein the correction function means includes means for determining the correction factor within a range of at least about ±

8 nanometers around the specified center wavelength.

17. The means of claim 15, wherein the one or more light sources comprise LEDs.

18. The system of claim 15, wherein at least one of the one or more light sources is an infrared light source and the correction function means includes means for determining {r} as a function of measured reflectance values R_IR in the infrared range and a constant r_IR that represents an average of R_IR, where each value in {r} equals the value of (R/R_IR)r_IR at a corresponding wavelength.

19. The system of claim 18, wherein the correction function means includes means for determining values of {r} at discrete wavelength intervals.

20. The system of claim 15, wherein wavelength correction means comprises a portion of a reflectometer means.

21. The wavelength correction means of claim 15, wherein the correction function means is configured to determine a correction factor

22. A reflectometer comprising:

A. a set of light sources;

B. a set of detectors;

C. a reflectance assembly configured to direct light signals from the set of light sources onto a test product and to direct light signals reflected from the test product onto the set of detectors;

D. at least one storage device configured to store a reference spectral distribution {L*}, a set of high resolution reflectance values {r}, a set of detector sensitivity data {D} corresponding to the set of detectors, a measured spectral distribution {L} corresponding tote set of light sources, and a set of measured reflectance values R; and

E. a correction function module for determining a correction factor at a given wavelength as a function of {L}, {L*}, {r} and {D} and to apply the correction factor to R to determine R*.

23. The reflectometer of claim 22, wherein the set of light sources comprises a set of LEDs.

24. The reflectometer of claim 22, wherein the correction function module is configured to determine a correction factor

25. A wavelength correction module, in a reflectance-based system comprising a set of light sources, a set of detectors, and a reflectance assembly configured to direct light signals from the set of light sources onto a test product and to direct light signals reflected from the test product onto the set of detectors, the wavelength correction module comprising:

A. at least one storage device configured to store a reference spectral distribution {L*}, a set of high resolution reflectance values {r}, a set of detector sensitivity data {D} corresponding to the set of detectors, a measured spectral distribution {L} corresponding to the set of light sources, and a set of measured reflectance values R; and

B. a correction function module for determining a correction factor at a given wavelengths a function of {L}, {L*}, {r} and {D} and to apply the correction factor to R to determine R*.

26. The wavelength correction module of claim 25, wherein the set of light sources comprises a set of LEDs.

27. The wavelength correction module of claim 25, wherein the correction function module is configured to determine a correction factor