Analysis system for reagent-free determination of the concentration of an analyte in living tissue
First Claim
1. A system for the reagent-free determination of the concentration of an analyte in living tissue of a patient, the system comprising:
- a light transmitter for generating monochromatic primary light having a wavelength of at least 550 nm and at most 900 nm;
a scattered-light percutaneous sensor adapted to be inserted through the skin surface into the skin, wherein a distal end of the percutaneous sensor has a sensor head, wherein a distal end of the sensor head is enclosed by a semipermeable membrane to define a test volume for containing interstitial fluid from the tissue, wherein the semipermeable membrane prevents admission of macromolecules having a molecular weight above the exclusion limit of the semipermeable membrane to the test volume;
an inbound light guide in which the primary light is conducted through the skin surface into the interior of the body;
a light irradiation surface formed at a distal end of the inbound light guide through which the primary light penetrates into the test volume;
a detection light guide which has at its distal end a light receiving surface through which a secondary light scattered in the test volume penetrates into the detection light guide, wherein the light irradiation surface of the inbound light guide and the light receiving surface of the detection light guide are adapted to be located subcutaneously so that the test volume contains the interstitial fluid;
a wavelength-sensitive detection device connected to the detection light guide for detection of Raman-scattered components of the secondary light; and
an evaluation device for determining the concentration of the analyte from the Raman-scattered components of the secondary light.
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Accused Products
Abstract
The present invention is generally directed towards a system for reagent-free determination of the concentration of an analyte in vivo. The system comprises a light transmitter for generating monochromatic primary light, a scattered-light percutaneous sensor which includes an inbound light guide and a detection light guide, a wavelength-selective detection device that is connected to the detection light guide for detection of Raman-scattered components of the secondary light and an evaluation device for determining the concentration of the analyte from the Raman-scattered components of the secondary light.
151 Citations
32 Claims
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1. A system for the reagent-free determination of the concentration of an analyte in living tissue of a patient, the system comprising:
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a light transmitter for generating monochromatic primary light having a wavelength of at least 550 nm and at most 900 nm; a scattered-light percutaneous sensor adapted to be inserted through the skin surface into the skin, wherein a distal end of the percutaneous sensor has a sensor head, wherein a distal end of the sensor head is enclosed by a semipermeable membrane to define a test volume for containing interstitial fluid from the tissue, wherein the semipermeable membrane prevents admission of macromolecules having a molecular weight above the exclusion limit of the semipermeable membrane to the test volume; an inbound light guide in which the primary light is conducted through the skin surface into the interior of the body; a light irradiation surface formed at a distal end of the inbound light guide through which the primary light penetrates into the test volume; a detection light guide which has at its distal end a light receiving surface through which a secondary light scattered in the test volume penetrates into the detection light guide, wherein the light irradiation surface of the inbound light guide and the light receiving surface of the detection light guide are adapted to be located subcutaneously so that the test volume contains the interstitial fluid; a wavelength-sensitive detection device connected to the detection light guide for detection of Raman-scattered components of the secondary light; and an evaluation device for determining the concentration of the analyte from the Raman-scattered components of the secondary light. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A system for the reagent-free determination of the concentration of an analyte in living tissue of a patient, the system comprising:
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a light transmitter for generating monochromatic primary light having a wavelength of at least 550 nm and at most 900 nm; a scattered-light percutaneous sensor adapted to be inserted through the skin surface into the skin, wherein a distal end of the percutaneous sensor has a sensor head, the sensor head defining a test volume for containing interstitial fluid from the tissue; an inbound light guide in which the primary light is conducted through the skin surface into the interior of the body; a light irradiation surface formed at a distal end of the inbound light guide through which the primary light penetrates into the test volume; a detection light guide which has at its distal end a light receiving surface through which a secondary light scattered in the test volume penetrates into the detection light guide, wherein the light inadiation surface of the inbound light guide and the light receiving surface of the detection light guide are adapted to be located subcutaneously so that the test volume contains the interstitial fluid; a wavelength-sensitive detection device connected to the detection light guide for detection of Raman-scattered components of the secondary light; an evaluation device for determining the concentration of the analyte from the Raman-scattered components of the secondary light; wherein the sensor head includes a reflective surface positioned around the test volume to reflect the Raman-scattered components of the secondary light towards the light receiving surface of the detection light guide; and wherein the reflective surface is positioned to not reflect the primary light emitted from the inbound light guide towards the light receiving surface of the detection light guide. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24)
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25. A method, comprising:
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inserting a sensor head of a scattered-light percutaneous sensor into skin, wherein the sensor head includes a light irradiation surface and a light receiving surface, wherein said inserting includes locating the light irradiation surface and the light receiving surface in subcutaneous connective tissue of the skin so that a test volume of the sensor head contains interstitial fluid, wherein the sensor head is enclosed by a semipermeable membrane to define the test volume; shining a monochromatic primary light that has wavelength of at least 550 nm and at most 900 nm from the light irradiation surface of the sensor head into the test volume containing the interstitial fluid; receiving a secondary light scattered from the test volume containing the interstitial fluid at the light receiving surface of the sensor head; detecting Raman-scattered components of the secondary light from the secondary surface with a wavelength-sensitive detection device; determining concentration of analyte in the test volume from the Raman-scattered components of the secondary light; and reducing fluorescence interference by preventing access of macromolecules that have a molecular weight greater than 50 kDa to the test volume with the semipermeable membrane of the sensor head. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32)
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Specification