Microfabricated reactor, process for manufacturing the reactor, and method of amplification

  • US 7,297,313 B1
  • Filed: 06/07/1995
  • Issued: 11/20/2007
  • Est. Priority Date: 08/31/1991
  • Status: Active Grant
First Claim
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1. A device for amplifying a preselected polynucleotide in a sample by conducting a polynucleotide amplification reaction, the device comprising:

  • a solid substrate which is microfabricated to define;

    a sample inlet port;

    a flow system for micro- to picoliter volumes, comprising;

    a sample flow channel extending from said inlet port; and

    a polynucleotide amplification reaction chamber in fluid communication with said flow channel;

    said chamber and said channel being of dissimilar dimension; and

    a fluid exit port in fluid communication with said flow system; and

    means for thermally cycling the contents of said chamber whereby in each cycle the temperature is controlled to dehybridize double stranded polynucleotide and to permit the in-vitro amplification of a preselected polynucleotide.

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