Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions

CAFC
  • US 7,312,039 B2
  • Filed: 10/31/2006
  • Issued: 12/25/2007
  • Est. Priority Date: 05/29/1996
  • Status: Expired due to Fees
First Claim
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1. A method for identifying a target nucleotide sequence comprising:

  • forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, wherein the ligation product comprises an upstream primer portion and a downstream primer portion, wherein the upstream primer portion and the downstream primer portion are not complementary with the target nucleotide sequence;

    amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, wherein the PCR mixture comprises an upstream primer and a downstream primer; and

    detecting the amplified ligation product to identify the target nucleotide sequence.

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