Device for the stepwise transport of liquid utilizing capillary forces

• US 7,316,802 B2
• Filed: 11/13/2003
• Issued: 01/08/2008
• Est. Priority Date: 11/14/2002
• Status: Active Grant

• Current Assignee: Boehringer Ingelheim Europe GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Original Assignee: Boehringer Ingelheim Europe GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Inventors: Blankenstein, Gert, Peters, Ralf-Peter
• Primary Examiner(s): Gordon; Brian R.
• Application Number:
  US10/706,028
• Publication Number:
  US 20040096358A1
• Time in Patent Office: 1,517 Days
• Field of Search: 422 99-101, 422/103, 422 57- 58
• US Class Current: 422/520
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2300/044 pierceable, e.g. films, membranes
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2300/087 Multiple sequential chambers
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0677 phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 2400/0694 vents used to stop and induce flow, backpressure valves
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001

Abstract

The device (10) for the stepwise transport of liquid, particularly of sample liquid to be analyzed, through several reaction chambers located in series in terms of flow while utilizing capillary forces comprises at least one channel (14) through which liquid is transportable on the basis of capillary forces. Further, the device (10) comprises at least two closed vent holes (38,40,42) which are in fluid communication with the channel (14) at connection sites (22,24,26) spaced from each other along the channel (14). The connection sites (22,24,26) divide the channel (14) into several channel sections (44,46,48). The fluid connections between a respective channel section (44,46,48) and the vent holes (38,40,42) allocated thereto can be opened separately.

Claims

1. A device for the stepwise transport of liquid, particularly of sample liquid to be analyzed, through several reaction chambers located in series in terms of flow while utilizing capillary forces, comprising

a channel (14) through which liquid is transportable on the basis of capillary forces, and

at least two closed vent holes (38,40,42) which are in fluid communication via respective fluid connections (30,32,34) with the channel (14) at connection sites (22,24,26) spaced from each other along the channel (14),

the connection sites (22,24,26) dividing the channel (14) into several channel sections (44,46,48),

the vent holes (38,40,42) allocated thereto being able to be opened separately, thereby allowing stepwise fluid flow into said respective channel sections (44,46,48), and

at least one chamber (50,52,54) being arranged in the channel sections (44,46,48) upstream of each connection site (22,24,26) in flow direction.

2. The device according to claim 1, characterized in that a reagent substance is arranged in at least one chamber (50,52,54).

3. The device according to claim 2, characterized in that the reagent substance is immobilized and adapted to be mobilized when contacting the liquid.

4. The device according to claim 1, characterized in that said fluid connections are vent channels (30,32,34) ending in the vent holes (38,40,42) and branch off from the channel (14) at the connection sites (22,24,26).

5. The device according to claim 4, characterized in that liquid is transportable through the vent channel (30,32,34) up to the vent hole (38,40,42) by capillary effect when the vent hole (38,40,42) is open.

6. The device according to claim 1 characterized in that a liquid flowing through the channel section (44,46,48) upstream of the vent hole (38,40,42) when viewed in flow direction after the vent hole (38,40,42) has been opened reaches up to the vent hole (38,40,42).

7. The device according to claim 1 characterized in that each vent hole (38,40,42) is closed by a cover element (60,74,76,78) that is adapted to be pulled off, punctured, melted open and/or soluble or air-permeable by initiating a reaction.

8. The device according to claim 7, characterized in that all the vent holes (38,40,42) are covered by a common cover element (60,74,76,78), the cover element (60,74,76,78) being adapted to be selectively pulled off, punctured, melted open and/or soluble or air-permeable by initiating a reaction.

9. The device according to claim 7 characterized in that one or more heating elements thermally coupled with the cover element (60,74,76,78) are provided for melting open the cover element (60,74,76,78).

10. The device according to claim 1 characterized in that several channels (14) are provided the first, second and further vent holes (38,40,42) of which, which succeed each other in flow direction, are respectively adapted to be uncovered in common in groups.

11. The device according to claim 1 characterized in that the vent holes (38,40,42) are capillary holes.

12. The device according to claim 8, characterized in that one or more heating elements thermally coupled with the cover element (60,74,76,78) are provided for melting open the cover element (60,74,76,78).