Use of vertically stacked photodiodes in a gene chip system

• US 7,316,930 B1
• Filed: 04/21/2003
• Issued: 01/08/2008
• Est. Priority Date: 04/21/2003
• Status: Active Grant

• Current Assignee: National Semiconductor Corporation (Texas Instruments, Inc.)
• Original Assignee: National Semiconductor Corporation (Texas Instruments, Inc.)
• Inventors: Montalbo, Joseph Domenick
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): JARRETT, LORE RAMILLANO
• Application Number:
  US10/421,647
• Time in Patent Office: 1,723 Days
• Field of Search: 436/172, 250/458.1, 438/1, 435/29, 435/288.4, 356/246
• US Class Current: 436/172
• CPC Class Codes: G01N 2021/0325 Cells for testing reactions, e.g. containing reagents
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: H01L 27/14627 Microlenses
• CPC Class Codes: H01L 27/14647 Multicolour imagers having a stacked pixel-element structure, e.g. npn, npnpn or MQW elements

Abstract

An improved gene chip system is arranged with vertically stacked photodiodes for sensing luminescence of genetic material. When genetic samples are combined with a reagent in an array of wells on a substrate, the reaction may cause light of a specified color to emanate from certain well locations. Vertically stacked photodiodes are provided that sense each color and intensity of the light at each well location. The vertically stacked photodiodes provide an image sensor that may be integrally constructed with the substrate and wells used for a gene chip system. The illumination data, which includes which wells are luminescent, the color of light, and the intensity of light in each illuminated cell, may be analyzed to determine properties of the genetic sample.

Claims

1. A gene chip system, comprising:

a substrate that includes an array of wells, wherein each of the wells receives and holds an amount of genetic sample and reagent; and

vertically stacked photodiodes, wherein each of the vertically stacked photodiodes are integrally constructed with the substrate and are located adjacent to and fixed below each one of the wells in the array of wells, and wherein each vertically stacked photodiodes senses light emanating from its corresponding well.

2. The gene chip system as in claim 1, wherein the substrate is one of a group comprising glass, nylon, and silicon.

3. The gene chip system as in claim 1, wherein each vertically stacked photodiode is sensitive to a particular color of light emanating from its respective well, and wherein particular colors include, but are not limited to, blue, green and red.

4. The gene chip system as in claim 1, wherein the vertically stacked photodiodes located adjacent to and corresponding to one of the array of wells comprise a blue-sensitive photodiode, a green-sensitive photodiode, and a red-sensitive photodiode.

5. The gene chip system as in claim 4, wherein the blue-sensitive diode is closest to the one of the array of wells when compared to the green-sensitive photodiode and a red-sensitive photodiode, the green-sensitive photodiode is further from the one of the array of wells when compared to the blue-sensitive photodiode and closer to the one of the array of wells when compared to the red-sensitive photodiode, and the red-sensitive photodiode is furthest from the one of the array of wells when compared to the green-sensitive photodiode and a blue-sensitive photodiode.

6. The gene chip system as in claim 1, wherein the substrate is a transparent material.

7. The gene chip system as in claim 6, wherein the vertically stacked photodiodes are positioned opposite the substrate from the array of wells included in the substrate, such that light emanating from illuminated wells within the array of wells is transmitted across the substrate to reach the vertically stacked photodiodes.

8. The gene chip system as in claim 1, wherein

the substrate has a first surface and a second surface opposite the first surface and separated therefrom by a thickness associated with the substrate,

each well is formed as a depressed region in the first surface having a thickness that is less than the thickness associated with the substrate in another region, and

the distance for light to travel between one of the array of wells and its corresponding vertically stacked photodiodes is less than the thickness associated with the substrate.

9. The gene chip system as in claim 1, wherein the vertically stacked photodiodes receive illumination data corresponding to a color and an intensity of light;

and wherein the gene chip system further comprises;

an analyzer for analyzing illumination data to determine properties related to the genetic sample.

10. The gene chip system as in claim 9, wherein the analyzer is arranged to output a digital image that illustrates the color and the intensity of light emanating from each illuminated well.

11. The gene chip system as in claim 1, wherein the vertically stacked photodiodes are integrally constructed in the substrate arranged for combining the genetic sample with a reagent.

12. The gene chip system as in claim 1, wherein the vertically stacked photodiodes are positioned on an opposite side of the substrate that is arranged to combine a genetic sample with a reagent from the array of wells, such that light emanating from an illuminated well is transmitted across this arrangement of the substrate to at least one of the vertically stacked photodiodes.