Non-affinity purification of proteins
DCFirst Claim
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1. A method for purifying a target protein from a mixture containing a host cell protein, consisting essentially of subjecting said mixture to:
- (a) a first non-affinity purification step, and(b) a second non-affinity purification step, followed by(c) high-performance tangential-flow filtration (HPTFF), and(d) isolating said protein in a purity containing less than 100 parts per million (ppm) of said host cell protein, wherein said method includes no affinity purification step.
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Abstract
The present invention relates to a method for protein purification that involves the combination of non-affinity chromatography with HPTFF.
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25 Claims
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1. A method for purifying a target protein from a mixture containing a host cell protein, consisting essentially of subjecting said mixture to:
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(a) a first non-affinity purification step, and (b) a second non-affinity purification step, followed by (c) high-performance tangential-flow filtration (HPTFF), and (d) isolating said protein in a purity containing less than 100 parts per million (ppm) of said host cell protein, wherein said method includes no affinity purification step. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25)
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Specification