Non-affinity purification of proteins

  • US 7,323,553 B2
  • Filed: 04/25/2003
  • Issued: 01/29/2008
  • Est. Priority Date: 04/26/2002
  • Status: Active Grant
First Claim
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1. A method for purifying a target protein from a mixture containing a host cell protein, consisting essentially of subjecting said mixture to:

  • (a) a first non-affinity purification step, and(b) a second non-affinity purification step, followed by(c) high-performance tangential-flow filtration (HPTFF), and(d) isolating said protein in a purity containing less than 100 parts per million (ppm) of said host cell protein, wherein said method includes no affinity purification step.

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