Immersion sensor for measuring the concentration of an analyte with the help of an oxidase

• US 7,335,286 B2
• Filed: 10/16/2003
• Issued: 02/26/2008
• Est. Priority Date: 04/18/2001
• Status: Expired due to Fees

• Current Assignee: Roche Diagnostics International AG (Roche Holding AG)
• Original Assignee: Disetronic Licensing Ag
• Inventors: Ehwald, Rudolf, Beyer, Uwe, Abel, Peter
• Primary Examiner(s): Lankford, Jr.; Leon B.
• Assistant Examiner(s): FERNANDEZ, SUSAN EMILY
• Application Number:
  US10/687,529
• Publication Number:
  US 20040229302A1
• Time in Patent Office: 1,594 Days
• Field of Search: 435/4, 435/14, 435/25, 435/177, 435/180, 435/287.1, 435/287.9, 20440301-40314, 600/347
• US Class Current: 204/403.01
• CPC Class Codes: C12Q 1/002 Electrode membranes
• CPC Class Codes: C12Q 1/006 for glucose

Abstract

The invention relates to measuring the concentration of analytes with the aid of an oxidase in an immersion sensor situated in a fluid or in a matrix containing fluid. Oxygen diffuses into the enzyme layer from within, from a gas-filled space connected to the atmosphere and/or to an oxygen reservoir. This enables oxygen saturation of the oxidase in a low-oxygen or oxygen-free medium and/or at high analyte concentrations. The analyte diffuses into the enzyme layer in a channel or a number of channels which contain(s) water and limit(s) diffusion, wherein the channel/channels on the surface of the sensor is/are filled with a protein-impermeable, hydrophilic matrix. By increasing the channel cross-section on the surface of the sensor and/or by connecting the channel/channels to a protein-impermeable, porous, hydrophilic layer on the surface of the sensor, the effect of outer deposits on the diffusion resistance of the analyte is reduced.

Claims

1. An immersion sensor for measuring the concentration of at least one analyte with the aid of an oxidase, wherein said immersion sensor comprises:

a base body wherein the outside surface of said base body is defined by an analyte-permeable region and an analyte-impermeable, oxygen-permeable region which is a membrane,

an enzyme region comprising an oxidase within said base body and connected to said membrane,

a pair of electrodes within said base body and adjacent to said enzyme region, and

at least one water-containing channel within said base body which connects the enzyme region to the analyte-permeable region of the outside surface of said base body.

2. The immersion sensor as set forth in claim 1, wherein the enzyme region contains water.

3. The immersion sensor as set forth in claim 1, wherein the at least one water-containing channel leads through an impermeable material in the base body of the immersion sensor.

4. The immersion sensor as set forth in claim 1, wherein said at least one channel is filled, adjacent to the surface of the sensor, with a porous substance which is impermeable to proteins.

5. The immersion sensor as set forth in claim 1, wherein said at least one water-containing channel contains a protein-impermeable, hydrophilic layer.

6. The sensor according to claim 1, wherein the sensor is configured such that the analyte diffuses into the enzyme region.

7. The sensor according to claim 1, wherein the enzyme region is an enzyme layer.

8. The sensor according to claim 1, wherein a length of the at least one water-containing channel exceeds a thickness of the membrane.

9. The sensor according to claim 1, wherein the enzyme region borders an inner gas space of the sensor from within.

10. The sensor according to claim 9, wherein the inner gas space is connected to an oxygen reservoir.

11. The sensor according to claim 9, wherein an oxygen-permeable membrane is situated between the enzyme layer and the inner gas space.

12. The sensor according to claim 1, wherein the at least one water-containing channel forms the only way of transporting analyte to the enzyme.

13. The sensor according to claim 1, wherein a diffusion resistance of the analyte in said at least one water-containing channel is determined by a ratio of a length of the at least one water-containing channel and a cross-sectional area of the at least one water-containing channel.

14. The sensor according to claim 1, wherein a length of the at least one water-containing channel is between 0.1 mm and 1 mm.

15. The sensor according to claim 1, wherein the analyte-impermeable, oxygen-permeable region is a porous layer, said porous layer providing an increased surface area for diffusion of the analyte into the at least one water-containing channel, whereby the concentration gradient of the analyte outside the surface of the sensor levels off reducing material deposits on the outside surface of the sensor thereby reducing the effect on diffusion flow of the analyte.

16. The sensor according to claim 1, wherein the at least one water-containing channel passes into a hydrophilic, porous and protein-excluding layer.

17. The sensor according to claim 1, wherein the at least one water-containing channel leads through a water-impermeable material and at the outside surface of the sensor is filled with a hydrophilic porous substance.

18. The sensor according to claim 1, wherein the membrane comprises a substantially continuous membrane.

19. An immersion sensor for measuring the concentration of at least one analyte with the aid of an oxidase, wherein said immersion sensor comprises:

a base body wherein the outside surface of said base body is defined by an analyte-permeable region and an analyte-impermeable, oxygen-permeable region which is a substantially continuous membrane,

an enzyme region comprising an oxidase within said base body and connected to said substantially continuous membrane,

a pair of electrodes within said base body and adjacent to said enzyme region; and

at least one water-containing channel within said base body which connects the enzyme region to the analyte-permeable region of the outside surface of said base body.

20. The sensor according to claim 19, wherein said membrane comprises a membrane having no analyte window or no unselective pores or perforations.