Peelable and resealable devices for biochemical assays

• US 7,371,563 B2
• Filed: 07/29/2002
• Issued: 05/13/2008
• Est. Priority Date: 11/08/2000
• Status: Expired due to Fees

• Current Assignee: Surface Logix, Inc.
• Original Assignee: Surface Logix, Inc.
• Inventors: Agosto, Melina Amber, Kim, Enoch, Kirk, Gregory L., Campbell, Stewart, Duffy, David, Schueller, Olivier
• Primary Examiner(s): Corcoran; Gladys J P
• Assistant Examiner(s): BOWERS, NATHAN ANDREW
• Application Number:
  US10/206,074
• Publication Number:
  US 20030032046A1
• Time in Patent Office: 2,115 Days
• Field of Search: 435/6, 435/287.2, 435/174, 435/180, 435/395, 435/288.4, 435/305.2, 435/288.3, 435/287.9, 435/287.8, 435/305.3, 435/305.1, 435/288.5, 156/99, 436/524, 436/527, 206/503, 101/127, 101/112, 428/132, 428/133, 428/135
• US Class Current: 435/288.5
• CPC Class Codes: B01D 2325/08 Patterned membranes
• CPC Class Codes: B01J 2219/00274 Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00662 Two-dimensional arrays within two-dimensional arrays
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: B01J 2219/00743 Cells
• CPC Class Codes: B01L 2200/021 Adjust spacings in an array of wells, pipettes or holders, format transfer between arrays of different size or geometry
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0829 Multi-well plates; Microtitration plates
• CPC Class Codes: B01L 2300/0848 Specific forms of parts of containers
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 3/5025 for parallel transport of multiple samples
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 5/00 : Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C12M 23/12 : Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12Q 1/025 : for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics antimicrobial activity C12Q1/18
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 : Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 15/1468 : with spatial resolution of the texture or inner structure of the particle
• CPC Class Codes: G01N 2015/1497 : Particle shape
• CPC Class Codes: G01N 33/54366 : Apparatus specially adapted for solid-phase testing
• CPC Class Codes: G06T 2207/30072 : Microarray; Biochip, DNA array; Well plate
• CPC Class Codes: G06T 7/0012 : Biomedical image inspection
• CPC Class Codes: G06T 7/70 : Determining position or orientation of objects or cameras camera calibration G06T7/80
• CPC Class Codes: Y10T 428/24281 : Struck out portion type
• CPC Class Codes: Y10T 428/24289 : Embedded or interlocked
• CPC Class Codes: Y10T 428/24306 : Diamond or hexagonal

Abstract

Devices and methods for performing assays on materials, particularly biological materials, are provided. The devices and methods make use of self-sealing members, which can be applied to a flat surface to form wells to facilitate immobilization of materials on the flat surface, then removed to yield a flat surface that facilitates the performance of processes on and/or detection of the immobilized material.

Claims

1. A kit for forming a device comprising:

a base plate;

a first removable member adapted to repeatedly self-seal on the base plate,

the first removable member defining at least one first orifice therein and having walls bounding the at least one first orifice on sides of the at least one first orifice,

the first removable member being in self-sealing contact with the base plate for defining at least one first well corresponding to a respective one of the at least one first orifice;

a second removable member adapted to repeatedly self-seal on at least one of the first removable member and the base plate,

the second removable member defining at least one second orifice therein and having walls bounding the at least one second orifice on sides of the at least one second orifice,

the second removable member being in self-sealing contact with one of the first removable member and the base plate for defining at least one second well corresponding to a respective one of the at least one second orifice,

wherein said at least one second orifice is smaller than said at least one first orifice; and

at least one registration element adapted to align the base plate, the first removable member, and the second removable member with one another.

2. The kit of claim 1, wherein the walls of the first removable member bounding the at least one first orifice are tapered in a direction from an upper surface of the first removable member toward a lower surface of the first removable member such that an opening of the at least one first orifice is larger in the upper surface of the first removable member than an opening of the at least one first orifice in the lower surface of the first removable member.

3. The kit of claim 1, wherein the walls of the second removable member bounding the at least one second orifice are tapered in a direction from an upper surface of the second removable member toward a lower surface of the second removable member such that an opening of the at least one second orifice is larger in the upper surface of the second removable member than an opening of the at least one second orifice in the lower surface of the second removable member.

4. The kit of claim 2, wherein the walls of the second removable member bounding the at least one second orifice are tapered in a direction from an upper surface of the second removable member toward a lower surface of the second removable member such that an opening of the at least one second orifice is larger in the upper surface of the second removable member than an opening of the at least one second orifice in the lower surface of the second removable member, a degree of tapering of the walls of the first removable member being substantially equal to a degree of tapering of the walls of the second removable member.

5. The kit of claim 1, wherein a contour of an opening of the at least one second orifice in a lower surface of the second removable member is substantially identical to a contour of a corresponding opening of the at least one first orifice in an upper surface of the first removable member.

6. The kit of claim 1, wherein at least one of the at least one first orifice and the at least one second orifice includes a plurality of orifices defining an array that spatially and dimensionally corresponds to a standard array of wells in an industry standard microtiter plate.

7. The kit of claim 6, wherein the standard microtiter plate is selected from the group consisting of a 6-well microtiter plate, a 12-well microtiter plate, a 24-well microtiter plate, a 96-well microtiter plate, a 384-well microtiter plate, a 1,536-well microtiter plate, and a 9,600-well microtiter plate.

8. The kit of claim 1, wherein the base plate is made of a material selected from the group consisting of glass, silicon, fused silica, metal films, polystyrene, poly(methylacrylate), and polycarbonate.

9. The kit of claim 1, further comprises a first layer of metal disposed on an upper surface of the base plate.

10. The kit of claim 9, wherein the first layer of metal made of a material is selected from the group consisting of gold, silver, platinum, palladium and copper.

11. The kit of claim 10, further comprising a second layer of metal disposed between the upper surface of the base plate and the first layer of metal.

12. The kit of claim 11, wherein the second layer of metal is made of a material selected from the group consisting of chromium and titanium.

13. The kit of claim 1, wherein the base plate is treated with a solution of mixed self-assembled monolayer (SAM).

14. The kit of claim 1, wherein the base plate is treated with a solution of biospecific mixed SAM.

15. The kit of claim 1, wherein the at least one registration element comprises a registration pin adapted to extend through the base plate, the first removable member, and the second removable member for aligning respective ones of the base plate, the first removable member and the second removable member.

16. The kit of claim 1, wherein the at least one registration element includes a visual mark disposed on at least one of the base plate, the first removable member and the second removable member.

17. The kit of claim 1, wherein at least one of the first removable member and the second removable member is made of polydimethylsiloxane (PDMS).

18. The kit of claim 1 wherein the base plate is treated to enhance immobilization of peptide substrates thereon.

19. The kit of claim 1 wherein the base plate is treated to enhance immobilization of kinase substrates thereon.

20. The kit of claim 1, wherein the base plate is treated to enhance immobilization of antibody substrates thereon.

21. The kit of claim 20, wherein the base plate is treated with a solution of mixed self-assembled monolayer (SAM), such that binding of kinase substrates is enhanced in discrete areas and reduced or eliminated in others.

22. The kit of claim 21, wherein the base plate is treated with a solution of mixed self-assembled monolayer (SAM), such that specific binding of kinase substrates in desired orientations is enhanced and non-specific binding of kinase substrates is reduced or eliminated.

23. The kit of claim 22, wherein the base plate is treated with mixed self-assembled monolayer (SAM) comprising about 0.1 to 20% maleimide-terminal groups in a background of tri(ethylene glycol) terminal groups.

24. A kit for forming a device comprising:

a base plate;

a first removable member adapted to repeatedly self-seal on the base plate,

the first removable member defining at least one first orifice therein and having walls bounding the at least one first orifice on sides of the at least one first orifice,

the first removable member being in self-sealing contact with the base plate for defining at least one first well corresponding to a respective one of the at least one first orifice;

a second removable member adapted to repeatedly self-seal on at least one of the first removable member and the base plate,

the second removable member defining at least one second orifice therein and having walls bounding the at least one second orifice on sides of the at least one second orifice,

the second removable member being in self-sealing contact with one of the first removable member and the base plate for defining at least one second well corresponding to a respective one of the at least one second orifice,

wherein said at least one second orifice is smaller than said at least one first orifice; and

at least one registration element adapted to align the base plate, the first removable member, and the second removable member with one another within 200 microns in all directions.

25. A kit for forming a device comprising:

a base plate;

a first removable member adapted to repeatedly self-seal on the base plate,

the first removable member defining at least one first orifice therein and having walls bounding the at least one first orifice on sides of the at least one first orifice,

the first removable member being in self-sealing contact with the base plate for defining at least one first well corresponding to a respective one of the at least one first orifice;

a second removable member adapted to repeatedly self-seal on at least one of the first removable member and the base plate,

the second removable member defining at least one second orifice therein and having walls bounding the at least one second orifice on sides of the at least one second orifice,

the second removable member being in self-sealing contact with one of the first removable member and the base plate for defining at least one second well corresponding to a respective one of the at least one second orifice,

wherein said at least one second orifice is smaller than said at least one first orifice; and

at least one registration element adapted to align the base plate, the first removable member, and the second removable member with one another in only one specific orientation.

26. The device of claim 25, wherein the at least one registration element comprises a first registration element, a second registration element, and a third registration element.

27. The device of claim 26, wherein the first registration element and second registration element are identical and the third registration element is different.

28. The device of claim 26, wherein the first registration element, the second registration element, and the third registration element are elliptical or circular cylinders of an approximately equal length.

29. The device of claim 28, wherein the first registration element has a first diameter, the second registration element has a second diameter, and the third registration element has a third diameter, wherein the first and second diameter are approximately equal, and are smaller than the third diameter.

30. The device of claim 26, further comprising in the base plate a first base registration orifice for the first registration pin, a second base registration orifice for the second registration pin, and a third base registration orifice for the third registration pin;

and

further comprising in the first removable member, a first member registration orifice for the first registration pin, a second member registration orifice for the second registration pin, and a third member registration orifice for the third registration pin,

wherein either or both of the first base registration orifice and the first member registration orifice, or the second base registration orifice and the second member registration orifice are not the same shape.

31. The device of claim 30, wherein one of the first base registration orifice and the first member registration orifice is oval or rectangular and the other one of the first base registration orifice and the first member registration orifice is circular.

32. The device of claim 31, wherein a short axis of the oval or rectangular orifice is approximately equal to a diameter of the circular orifice.

33. The device of claim 30, wherein one of the second base registration orifice and the second member registration orifice is oval or rectangular and the other one of the second base registration orifice and the second member registration orifice is circular.

34. The device of claim 33, wherein a short axis of the oval or rectangular orifice is approximately equal to a diameter of the circular orifice.

35. The device of claim 30, wherein the third base registration orifice and the third member registration orifice are substantially identical in size and shape.

36. The device of claim 26, further comprising in the base plate a first base registration orifice for the first registration pin, a second base registration orifice for the second registration pin, and a third base registration orifice for the third registration pin;

and

further comprising in the first removable member, a first member registration orifice for the first registration pin, a second member registration orifice for the second registration pin, and a third member registration orifice for the third registration pin,

the first base registration orifice is oval or rectangular, and has a first long axis and a first short axis,

the second base registration orifice is oval or rectangular, and has a second long axis and a second short axis,

the third base registration orifice is oval or rectangular, and has a third long axis and a third short axis,

wherein the first long axis is parallel to the second long axis, and perpendicular to the third long axis.

37. The device of claim 26, further comprising in the base plate a first base registration orifice for the first registration pin, a second base registration orifice for the second registration pin, and a third base registration orifice for the third registration pin;

and

further comprising in the first removable member, a first member registration orifice for the first registration pin, a second member registration orifice for the second registration pin, and a third member registration orifice for the third registration pin,

the first member registration orifice is oval or rectangular, and has a first long axis and a first short axis,

the second member registration orifice is oval or rectangular, and has a second long axis and a second short axis,

the third member registration orifice is oval or rectangular, and has a third long axis and a third short axis,

wherein the first long axis is parallel to the second long axis, and perpendicular to the third long axis.