Electrodes linked via oligomers to nucleic acids

• US 7,381,533 B2
• Filed: 12/06/2005
• Issued: 06/03/2008
• Est. Priority Date: 11/05/1996
• Status: Expired due to Fees

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Clinical Micro Sensors Inc. (Roche Holding AG)
• Inventors: O'Connor, Stephen D., Meade, Thomas J., Yu, Changjun, Kayyem, Jon F., Gozin, Michael
• Primary Examiner(s): WHISENANT, ETHAN C
• Application Number:
  US11/295,993
• Publication Number:
  US 20060099631A1
• Time in Patent Office: 910 Days
• Field of Search: 435/6, 536/23.1, 536/24.3
• US Class Current: 435/6.11
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors

Abstract

The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them.

Claims

1. A composition comprising:

a) an electrode comprising;

i) a monolayer; and

ii) a first nucleic acid covalently attached to said electrode via a spacer; and

b) a second nucleic acid comprising at least one electron transfer moiety (ETM) covalently attached to said second nucleic acid.

2. A composition according to claim 1 wherein said spacer is a conductive oligomer.

3. A compositions according to claim 1 wherein said spacer is an insulator.

4. A composition according to claim 1 wherein said spacer comprises alkyl chains.

5. A composition according to claim 1 wherein said second nucleic acid comprises a plurality of ETMs.

6. A composition according to claim 1 wherein said ETM is a metallocene.

7. A composition according to claim 6 wherein said metallocene is ferrocene.

8. A composition according to claim 7 wherein said ferrocene is a ferrocene derivative.

9. A composition according to claim 1 wherein said monolayer comprises conductive oligomers.

10. A composition according to claim 1 wherein said monolayer comprises insulators.

11. A composition according to claim 1 further comprising a target sequence, wherein said target sequence, said first nucleic acid, and said second nucleic acid form a hybridization complex.

12. A composition according to claim 11 wherein said target sequence comprises a first domain and a second domain, wherein said first nucleic acid hybridizes to said first domain and said second nucleic acid hybridizes to said second domain.