Electrophoresis apparatus and a plate therefor

• US 7,384,527 B2
• Filed: 08/01/2001
• Issued: 06/10/2008
• Est. Priority Date: 08/01/2000
• Status: Expired due to Fees

• Current Assignee: Sensis Pty Limited (Telstra Group Ltd.)
• Original Assignee: Sensis Pty Limited (Telstra Group Ltd.)
• Inventors: Weissman, Zeev, Goldberg, Doron, Shapiro, Menachem, Ruschin, Shlomo
• Primary Examiner(s): Phasge; Arun S.
• Application Number:
  US10/343,633
• Publication Number:
  US 20040007465A1
• Time in Patent Office: 2,505 Days
• Field of Search: 204/600, 204/603, 204/606, 204/612, 204/615, 204/616
• US Class Current: 204/606
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44721 by optical means
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

The invention provides an electrophoresis apparatus comprising an electrophoretic chamber containing at least on two-plate housing for a sieving matrix, each said at least one two-plate housing consisting of a cover plate and a plate for housing said sieving matrix, wherein said plate includes an optical waveguide array which is characterized by being aligned with respect to a reference direction such as to enable one or more light beams incident on the sieving matrix along said reference direction to be maintained well-confined along said reference direction within any desired length of interaction along the width of said sieving matrix.

Claims

1. A plate for housing a sieving matrix for electrophoresis of a sample, said plate including an optical waveguide array within the sieving matrix, said optical waveguide array being adapted to focus and/or refocus a laser beam, and being characterized by being aligned with respect to a reference direction such as to enable one or more laser beams incident on the sieving matrix along said reference direction to be maintained well-confined along said reference direction within any desired length of interaction along the width of said sieving matrix.

2. A plate according to claim 1, wherein said optical waveguide array contains one sole waveguide.

3. A plate according to claim 1, wherein said optical waveguide array contains a plurality of waveguides.

4. A plate according to claim 1, wherein said optical waveguide comprises at least one waveguiding unit, each said waveguiding unit characterized in enabling diffracted laser beam in the sieving matrix along said reference direction, incident with respect to said waveguiding unit, to be refocused along said reference direction, thereby maintaining a well-confined laser beam within at least a portion of said sieving matrix along said reference direction.

5. A plate according to claim 1, wherein at least one said waveguiding unit comprises at least one convex-shaped lens.

6. A plate according to claim 1, wherein said one or more laser beams incident on the sieving matrix are collimated by a collimator located before the first waveguiding unit.

7. A plate according to claim 6, wherein said collimator is a convex-shaped lens.

8. A two-plate housing for a sieving matrix for electrophoresis of a sample comprising a plate according to claim 1, and a cover plate.

9. An electrophoresis apparatus comprising:

(i) an electrophoretic chamber containing at least one two-plate housing for a sieving matrix, each said at least one two-plate housing consisting of a cover plate and a plate for housing said sieving matrix, wherein said plate includes an optical waveguide array within the sieving matrix, wherein said optical waveguide is adapted to refocus a laser beam and which is characterized by being aligned with respect to a reference direction such as to enable the laser beam incident on the sieving matrix along said reference direction to be maintained well-confined along said reference direction within any desired length of interaction along the width of said sieving matrix;

(ii) at least one laser source; and

(iii) at least one light detector.

10. An electrophoresis apparatus according to claim 9, wherein said optical waveguide array contains one sole waveguide.

11. An electrophoresis apparatus according to claim 9, wherein said optical waveguide array contains a plurality of waveguides.

12. An electrophoresis apparatus according to claim 9, wherein each said optical waveguide comprises at least one waveguiding unit, each said waveguiding unit characterized in enabling diffracted laser beam in the sieving matrix along said reference direction, incident with respect to said waveguiding unit, to be refocused along said reference direction, thereby maintaining a well-confined laser beam within at least a portion of said sieving matrix along said reference direction.

13. An electrophoresis apparatus according to claim 12, wherein at least one said waveguiding unit is a convex-shaped lens.

14. An electrophoresis apparatus according to claim 9, wherein said one or more laser beams incident on the sieving matrix are collimated by a collimator located before the first waveguiding unit.

15. An electrophoresis apparatus according to claim 14, wherein said collimator is a convex-shaped lens.

16. An electrophoresis apparatus according to claim 9, wherein said at least one laser source comprises means for generating a plurality of laser beams.

17. An electrophoresis apparatus according to claim 16, wherein each one of said plurality of said laser beams is of a substantially different wavelength.

18. A plate including a sieving matrix for electrophoresis of a sample,

said plate including an optical waveguide array extending in the width direction of the plate within the sieving matrix for focusing a laser beam,

said optical waveguide array comprising a plurality of convex shaped lenses extending along said array,

said array being characterized by being aligned with respect to a reference direction such as to enable the laser beam incident on the sieving matrix along said reference direction to be maintained focused and well-confined along said reference direction within any desired length of interaction along the width of said sieving matrix.

19. A generally planar plate extending along x and y directions, wherein the x direction is the width direction, and the y direction is the length direction, said plate including a sieving matrix for electrophoresis of a sample,

said plate including an optical waveguide array within the sieving matrix having a plurality of gel lanes extending in the y direction,

said optical waveguide array being aligned in the x direction such as to enable a laser beam incident on the sieving matrix along the x direction to be maintained focused and well-confined along said x direction within any desired length of interaction along the width of said sieving matrix.