Electrodes linked via conductive oligomers to nucleic acids

• US 7,384,749 B2
• Filed: 01/30/2006
• Issued: 06/10/2008
• Est. Priority Date: 11/05/1996
• Status: Expired due to Fees

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Clinical Micro Sensors Inc. (Roche Holding AG)
• Inventors: Gozin, Michael, O'Connor, Stephen D., Meade, Thomas J., Yu, Changjun, Kayyem, Jon F.
• Primary Examiner(s): WHISENANT, ETHAN C
• Application Number:
  US11/343,462
• Publication Number:
  US 20060211016A1
• Time in Patent Office: 862 Days
• Field of Search: 435/6, 536/23.1, 536/24.3
• US Class Current: 435/6.11
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 40/00 Manufacture or treatment of nanostructures
• CPC Class Codes: C07H 21/02 with ribosyl as saccharide radical
• CPC Class Codes: C07H 21/04 with deoxyribosyl as saccharide radical
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors

Abstract

The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them.

Claims

1. A method of detecting a target nucleic acid sequence in a nucleic acid sample comprising

a) providing a solid support comprising an array of electrodes each comprising:

i) a covalently attached anchor sequence; and

ii) a self-assembled monolayer;

b) contacting said array with at least one target sequence and a second nucleic acid sequence comprising a first region substantially complementary to said anchor sequence and a second region substantially complementary to a region of said target sequence, to form a hybridization complex;

wherein said hybridization complex further comprises an electron transfer moiety (ETM); and

c) detecting the presence of said ETM as an indication of the presence of said target nucleic acid sequence.

2. A method according to claim 1 wherein said ETM is covalently attached to said target sequence.

3. A method according to claim 1 wherein said hybridization complex further comprises a label probe comprising said ETM.

4. A method according to claim 1 wherein said hybridization complex comprises a plurality of ETMs.

5. A method according to claim 1 wherein said ETM is a metallocene.

6. A method according to claim 5 wherein said metallocene is ferrocene.

7. A method according to claim 6 wherein said ferrocene is a ferrocene derivative.

8. A method according to claim 1 wherein said self-assembled monolayer comprises conductive oligomers.

9. A method according to claim 1 wherein said self-assembled monolayer comprises insulators.

10. A method according to claim 1 where said covalent attachment is via a spacer.

11. A method according to claim 10 wherein said spacer is a conductive oligomer.

12. A method according to claim 10 wherein said spacer is an insulator.