Sensor chips with multiple layers of polysiloxane

• US 7,390,649 B2
• Filed: 07/24/2001
• Issued: 06/24/2008
• Est. Priority Date: 07/27/2000
• Status: Expired due to Fees

• Current Assignee: Safeguard Biosystems Holdings Ltd.
• Original Assignee: Micronas Holding GmbH (TDK Corporation)
• Inventors: Mohry, Sonia, Ruhe, Jurgen, Klapproth, Holger
• Primary Examiner(s): Zimmer, Marc S
• Application Number:
  US10/343,024
• Publication Number:
  US 20040081835A1
• Time in Patent Office: 2,527 Days
• Field of Search: 427/387, 428/447, 428/426, 428/446, 428/702, 106/287.13, 106/287.16
• US Class Current: 435/287.1
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: Y10T 428/31663 As siloxane, silicone or silane

Abstract

The invention relates to sensor chips with multiple layers of polysiloxane with improve homogeneity.

Claims

1. A method of making a sensor chip comprising the steps of:

(a) providing a carrier disposed in the sensor chip, and having a surface of metal oxide or metalloid oxide; and

(b) homogenously layering a multiple layer of polysiloxane to the surface of the carrier, wherein the multiple layer of polysiloxane is obtained by a process comprising the steps of;

i. immersing the carrier in a solution of a single bi-functional silane linker having a concentration in the range of from 0.1 to 50 weight % in an organic solvent, wherein a first functionality of the bi-functional silane linker comprising two or three hydrolysable atoms or groups on a silicon atom enables crosslinking, and wherein a second functionality of the bi-functional silane linker enables immobilization of sample or probe molecules; and

ii. withdrawing the carrier at a speed in the range of from 0.1 to 10 mm/s, wherein bi-functional silane is fixed on the surface of the sensor chip by formation of a homogeneous multiple layer of polysiloxane through cross-linking, wherein the second functionality of the bi-functional silane linker is capable of undergoing nucleophilic substitution reactions, nucleophilic addition reactions, Diels-Alder reactions or radical substitution reactions.

2. A method as recited in claim 1, wherein the thickness of the multiple layer of polysiloxane is regulated by a speed of withdrawing the carrier.

3. A method as recited in claim 1, wherein silation of the carrier occurs over a period of 5 minutes.

4. A method as recited in claim 3, wherein fixing of the multiple layer of polysiloxane is performed at 100°

C. for 2 hours.

5. A method as recited in claim 3, wherein fixing of the multiple layer of polysiloxane is performed at 100°

C.-120°

C. for 2 hours.

6. A method as recited in claim 1, wherein the solvent has a boiling temperature in the range of from 50-150°

C.

7. A sensor chip, comprising:

(a) a carrier having a surface of metal oxide or metalloid oxide;

(b) a multiple layer of polysiloxane homogenously layered onto the carrier surface, wherein the multiple layer of polysiloxane is obtained by a process comprising the steps of

i. immersing the carrier in a solution of a single bi-functional silane linker having a concentration in the range of from 0.1 to 50 weight % in an organic solvent, wherein a first functionality of the bi-functional silane linker comprising two or three hydrolysable atoms or groups on a silicon atom enables crosslinking, and wherein a second functionality of the bi-functional silane linker enables immobilization of sample or probe molecules; and

ii. withdrawing the carrier at a speed in the range of from 0.1 to 10 mm/s, whereby bi-functional silane is fixed on the surface of the sensor chip by formation of a homogeneous multiple layer of polysiloxane through cross-linking; and

(c) a sample or probe molecule immobilized by the second functionality of the bi-functional silane linker.

8. A method of making a sensor chip comprising the steps of:

(a) providing a carrier having a surface of metal oxide or metalloid oxide; and

(b) homogenously layering a multiple layer of polysiloxane to the surface of the carrier, wherein the multiple layer of polysiloxane is obtained by a process comprising the steps of;

i. immersing the carrier in a solution of a single bi-functional silane linker having a concentration in the range of from 0.1 to 50 weight % in an organic solvent, wherein a first functionality of the bi-functional silane linker comprising two or three hydrolysable atoms or groups on a silicon atom enables crosslinking, and wherein a second functionality of the bi-functional silane linker enables immobilization of sample or probe molecules; and

ii. withdrawing the carrier at a speed in the range of from 0.1 to 10 mm/s, wherein bi-functional silane is fixed on the surface of the sensor chip by formation of a homogeneous multiple layer of polysiloxane through cross-linking; and

(c) immobilizing a sample or probe molecule using the second functionality of the bi-functional silane linker.