Device for detecting emission light of micro-object
First Claim
1. A micro-object emission light detecting device for detecting with a semiconductor light detecting element emission light emitted in the form of fluorescence or phosphorescence from a micro-object irradiated with excitation light emitted from an excitation light source;
- wherein said semiconductor light detecting element and said excitation light source are disposed coaxially or on one side, said micro-object emission light detecting device comprising;
a Q switch laser that generates a short pulse laser in response to the irradiation with the excitation light, elevates a peak light intensity instantaneously, and irradiates the micro-object with a beam of short pulse laser as the excitation light; and
a converging microlens that is inserted partway along a light path of the excitation light to converge the excitation light and elevate a peak light intensity of the excitation light and that irradiates the micro-object with the excitation light having the peak light intensity elevated;
whereby emission light is generated from the micro-object due to two-photon absorption and detected with the semiconductor light detecting elementwherein said Q switch laser is a passive type comprising a gain medium and a saturable absorber, andwherein the gain medium of said Q switch laser is a semiconductor with quantum well structure and the saturable absorber of said Q switch laser is also a semiconductor with quantum well structure.
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Abstract
In the detection of fluorescence Lf emitted by a micro-object irradiated with an excitation light Le by a semiconductor light-detecting element 20, a converging microlens 62 for converging the excitation light Le elevating the optical density thereof and irradiating the micro-object with the light, causing the micro-object to generate fluorescence Lf due to two-photon absorption, is inserted partway along the light path of the excitation light Le. This enables the fluorescence Lf emitted by the micro-object to be detected with high sensitivity.
19 Citations
15 Claims
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1. A micro-object emission light detecting device for detecting with a semiconductor light detecting element emission light emitted in the form of fluorescence or phosphorescence from a micro-object irradiated with excitation light emitted from an excitation light source;
- wherein said semiconductor light detecting element and said excitation light source are disposed coaxially or on one side, said micro-object emission light detecting device comprising;
a Q switch laser that generates a short pulse laser in response to the irradiation with the excitation light, elevates a peak light intensity instantaneously, and irradiates the micro-object with a beam of short pulse laser as the excitation light; and a converging microlens that is inserted partway along a light path of the excitation light to converge the excitation light and elevate a peak light intensity of the excitation light and that irradiates the micro-object with the excitation light having the peak light intensity elevated; whereby emission light is generated from the micro-object due to two-photon absorption and detected with the semiconductor light detecting element wherein said Q switch laser is a passive type comprising a gain medium and a saturable absorber, and wherein the gain medium of said Q switch laser is a semiconductor with quantum well structure and the saturable absorber of said Q switch laser is also a semiconductor with quantum well structure. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
- wherein said semiconductor light detecting element and said excitation light source are disposed coaxially or on one side, said micro-object emission light detecting device comprising;
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15. A micro-object emission light detecting device for detecting with a semiconductor light detecting element emission light emitted in the form of fluorescence or phosphorescence from a micro-object irradiated with excitation light emitted from an excitation light source;
- wherein said semiconductor light detecting element and said excitation light source are disposed coaxially or on one side, said micro-object emission light detecting device comprising;
a Q switch laser that generates a short pulse laser in response to the irradiation with the excitation light, elevates a peak light intensity instantaneously, and irradiates the micro-object with a beam of short pulse laser as the excitation light; and a converging microlens that is inserted partway along a light path of the excitation light to converge the excitation light and elevate a peak light intensity of the excitation light and that irradiates the micro-object with the excitation light having the peak light intensity elevated; whereby emission light is generated from the micro-object due to two-photon absorption and detected with the semiconductor light detecting element, wherein said Q switch laser is a passive type comprising a gain medium and a saturable absorber wherein the gain medium of said Q switch laser and the saturable absorber are quantum wells formed of InGaAs grown on a GaAs substrate, and the composition of the In and the Ga and the thickness of the InGaAs quantum well layer is so fixed as to give rise to an overlap between the gain curve of said gain medium and the absorption band of said saturable absorber.
- wherein said semiconductor light detecting element and said excitation light source are disposed coaxially or on one side, said micro-object emission light detecting device comprising;
Specification