Fluid based analysis of multiple analytes by a sensor array

• US 7,491,552 B2
• Filed: 01/20/2005
• Issued: 02/17/2009
• Est. Priority Date: 07/16/1998
• Status: Expired due to Fees

• Current Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Inventors: Neikirk, Dean P., Anslyn, Eric V., McDevitt, John T., Shear, Jason B.
• Primary Examiner(s): Le; Long V
• Assistant Examiner(s): BROWN, MELANIE YU
• Application Number:
  US11/039,054
• Publication Number:
  US 20050164320A1
• Time in Patent Office: 1,489 Days
• Field of Search: 436/518, 436/164, 436/501, 436/523, 436/524, 436/800, 436/805, 422/68.1, 422/81, 422/82.01, 422/243, 422/82.07, 435/287.1, 435/287.2, 435/288.4, 435/288.7, 356/38, 356/45, 356/73, 356/311, 356/335
• US Class Current: 436/518
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00648 by the use of solid beads
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 21/77 by observing the effect on a chemical indicator
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus
• CPC Class Codes: Y10S 435/973 Simultaneous determination of more than one analyte
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine
• CPC Class Codes: Y10S 436/805 Optical property
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays
• CPC Class Codes: Y10T 436/25 including sample preparation

Abstract

A system for the rapid characterization of multi-analyte fluids, in one embodiment, includes a light source, a sensor array, and a detector. The sensor array is formed from a supporting member into which a plurality of cavities may be formed. A series of chemically sensitive particles are, in one embodiment positioned within the cavities. The particles may be configured to produce a signal when a receptor coupled to the particle interacts with the analyte. Using pattern recognition techniques, the analytes within a multi-analyte fluid may be characterized.

Claims

1. A sensor array for detecting multiple analytes in a fluid comprising:

a supporting member, wherein a plurality of cavities are formed within the supporting member and the cavities extend through the supporting member;

a plurality of particles positioned within the plurality of cavities, wherein the particles are configured to produce a signal when the particle interacts with an analyte;

a cover positioned above the supporting member and configured to inhibit dislodgment of the particle during use; and

one or more support structures coupled to the supporting member and to the cover;

wherein the one or more support structures space the cover apart from the supporting member by a fixed distance to define a channel therebetween, and wherein the fluid enters the cavity via the channel during use.

2. The sensor array of claim 1, wherein the particle is composed of a polymeric resin, and further comprising a receptor molecule coupled to the polymeric resin.

3. The sensor array of claim 1, wherein the particle has a size ranging from about 0.05 microns to about 500 microns in diameter.

4. The sensor array of claim 1, wherein the particle has a size ranging from about 0.05 microns to bout 500 microns in diameter, and wherein the cavity is configured to substantially contain the particle.

5. The sensor array of claim 1, wherein the supporting member comprises a plastic material.

6. The sensor array of claim 1, wherein the supporting member comprises a silicon wafer.

7. The sensor array of claim 6, wherein the cavities are substantially pyramidal in shape and wherein the sidewalls of the cavities are substantially tapered at an angle from about 50 degrees to about 60 degrees.

8. The sensor array of claim 1, further comprising channels in the supporting member, wherein the channels are configured to allow the fluid to flow through the channels into and away from the cavities.

9. The sensor array of claim 1, further comprising an inner surface coating wherein the inner surface coating is configured to inhibit dislodgment of the particle.

10. The sensor array of claim 1, further comprising a detector coupled to the bottom surface of the supporting member, wherein the detector is positioned below the cavities.

11. The sensor array of claim 1, wherein the cover is substantially transparent.

12. The sensor array of claim 11, wherein the cover comprises plastic, glass, quartz, silicon oxide, or silicon nitride.

13. The sensor array of claim 1, wherein at least one of the support structures comprises a polymer material or a silicon-based material.