Device for chemical and biochemical reactions using photo-generated reagents
First Claim
1. A method for synthesizing one or more selected multimers at specific reaction sites on a substrate, the specific reaction sites containing one or more protected initiation moieties, the method comprising:
- (a) contacting the substrate with a liquid solution comprising one or more photo-reagent precursors, said precursors selected from the group consisting of acid precursors and base precursors, such that the liquid solution is in contact with the initiation moieties;
(b) fluidly isolating the specific reaction sites;
(c) irradiating a selected number of the isolated reaction sites, without use of a photolithographic mask, with an optical system comprising a light source and a computer-controlled spatial optical modulator comprising a digital micromirror device to produce at least one photo-generated reagent that directly deprotects the initiation moieties at the irradiated reaction sites so as to create deprotected initiation moieties;
(d) contacting the substrate with one or more monomers, the monomers comprising an unprotected reactive site and a protected reactive site, under conditions such that the unprotected reactive site of the monomers couple with the deprotected initiation moieties so as to create new initiation moieties with attached monomers; and
(e) repeating steps (a)-(d) until the selected multimers are synthesized.
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Abstract
This invention provides method and apparatus for performing chemical and biochemical reactions in solution using in situ generated photo-products as reagent or co-reagent. In some embodiments, the present invention provides methods and devices for generating one or more selected multimers at specific reaction sites on a substrate comprising contacting the substrate with a liquid solution comprising photo-reagent precursors, isolating the reaction sites, selectively irradiating the reaction sites with a digital micromirror device, and contacting the substrate with monomers. The method and apparatus of the present invention have applications in parallel synthesis of molecular sequence arrays on solid surfaces.
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Citations
30 Claims
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1. A method for synthesizing one or more selected multimers at specific reaction sites on a substrate, the specific reaction sites containing one or more protected initiation moieties, the method comprising:
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(a) contacting the substrate with a liquid solution comprising one or more photo-reagent precursors, said precursors selected from the group consisting of acid precursors and base precursors, such that the liquid solution is in contact with the initiation moieties; (b) fluidly isolating the specific reaction sites; (c) irradiating a selected number of the isolated reaction sites, without use of a photolithographic mask, with an optical system comprising a light source and a computer-controlled spatial optical modulator comprising a digital micromirror device to produce at least one photo-generated reagent that directly deprotects the initiation moieties at the irradiated reaction sites so as to create deprotected initiation moieties; (d) contacting the substrate with one or more monomers, the monomers comprising an unprotected reactive site and a protected reactive site, under conditions such that the unprotected reactive site of the monomers couple with the deprotected initiation moieties so as to create new initiation moieties with attached monomers; and (e) repeating steps (a)-(d) until the selected multimers are synthesized. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 25, 26, 27, 28, 29, 30)
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15. A method for synthesizing, in solution, one or more selected multimers at specific reaction sites on a substrate, the specific reaction sites containing one or more protected initiation moieties, the method comprising:
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(a) contacting the substrate with a liquid solution comprising one or more photo-generated reagents, wherein said photogenerated reagents are generated, without use of a photolithographic mask, using an optical system comprising a light source and a computer-controlled spatial optical modulator comprising a digital micromirror device to irradiate specific, fluidly isolated reaction sites, such that the liquid solution is in contact with a selected number of initiation moieties, thereby directly deprotecting the initiation moieties so as to create deprotected initiation moieties; (b) contacting the substrate with one or more monomers, the monomers comprising an unprotected reactive site and a protected reactive site, under conditions such that the unprotected reactive site of the monomers couple with the deprotected initiation moieties so as to create new initiation moieties with attached monomers; and (c) repeating steps (a)-(b) until the selected multimers are synthesized. - View Dependent Claims (16, 17, 18, 19, 20, 21, 22, 23, 24)
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Specification