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Detection of analytes using reorganization energy

  • US 7,514,228 B2
  • Filed: 08/02/2007
  • Issued: 04/07/2009
  • Est. Priority Date: 06/12/1997
  • Status: Expired due to Fees
First Claim
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1. A method of detecting a target analyte in a test sample comprising:

  • a) adding a target analyte to an electrode comprising;

    i) a redox active molecule with a first E0, said redox active molecule comprising a metal selected from the group consisting of manganese, technetium, rhenium, iron, ruthenium, osmium, cobalt, rhodium, iridium, nickel, palladium, platinum, copper, silver and gold; and

    ii) a binding ligand that will bind said target analyte;

    such that upon binding of said target analyte to said binding ligand a redox active complex with a second different E0 is formed; and

    b) measuring said second E0 as an indication of the presence of said target analyte.

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