Isolation and transplantation of retinal stem cells

• US 7,514,259 B2
• Filed: 02/12/2001
• Issued: 04/07/2009
• Est. Priority Date: 02/11/2000
• Status: Expired due to Fees

• Current Assignee: Children's Hospital of Orange County, The Schepens Eye Research Institute Inc (Mass General Brigham, Inc.)
• Original Assignee: Schepens Eye Research Institute
• Inventors: Klassen, Henry J., Young, Michael J., Shatos, Marie A., Mizumoto, Keiko
• Primary Examiner(s): Hama; Joanne
• Application Number:
  US10/203,105
• Publication Number:
  US 20030207450A1
• Time in Patent Office: 2,976 Days
• Field of Search: 435/325, 800/8
• US Class Current: 435/366
• CPC Class Codes: A01K 67/0271 Chimeric vertebrates, e.g. comprising exogenous cells
• CPC Class Codes: A61K 35/12 Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells vaccines or medicinal preparations containing antigens or antibodies A61K39/00
• CPC Class Codes: A61P 27/02 Ophthalmic agents
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: C12N 2501/11 Epidermal growth factor [EGF]
• CPC Class Codes: C12N 2501/115 Basic fibroblast growth factor (bFGF, FGF-2)
• CPC Class Codes: C12N 2501/135 Platelet-derived growth factor [PDGF]
• CPC Class Codes: C12N 2506/02 from embryonic cells
• CPC Class Codes: C12N 5/0621 Eye cells, e.g. cornea, iris pigmented cells photoreceptors C12N5/062
• CPC Class Codes: C12N 5/0623 Stem cells

Abstract

The present invention relates to the isolation, in vitro propagation, and transplantation and integration of non-pigmented retinal stem cells derived from the neuroretina of the eye, ex vivo and in vivo.

Claims

1. A method of repopulating photoreceptor cells of a mammal, said method comprising the steps of:

providing neuroretina-derived retinal stem cells obtained from a neurosphere from a mammalian donor; and

introducing said neuroretina-derived retinal stem cells locally into a retinal site, a subretinal space, or a vitreal cavity of an eye of a mammalian recipient, wherein said donor mammal and said recipient mammal are allogeneic or syngeneic, and whereby said photoreceptor cells repopulate a mammalian retina.

2. The method of claim 1, wherein the neuroretinal-derived retinal stem cells are clonally derived.

3. The method of claim 1, wherein the mammal is a human.

4. The method of claim 1, wherein the donor and the recipient are allogeneic.

5. The method of claim 1, wherein the donor and recipient are syngeneic.

6. The method of claim 3, wherein the human recipient is a neonate.

7. The method of claim 3, wherein the human recipient is an adult.