Automated process for detecting the presence of a target nucleic acid in a sample
DCFirst Claim
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1. A process for detecting the presence of a target nucleic acid in a sample containing the target nucleic acid, the process comprising performing the automated steps of:
- (a) immobilizing the target nucleic acid on a solid support comprising a magnetically-responsive particle;
(b) subjecting the solid support to a magnetic field and aspirating non-immobilized components of the sample;
(c) performing an amplification reaction using the target nucleic acid immobilized in step (a) to produce an amplification product indicative of the presence of the target nucleic acid in the sample; and
(d) detecting the amplification product of step (c) as an indication of the presence of the target nucleic acid in the sample,wherein the steps of the process are performed in a self-contained analyzer.
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Abstract
An automated process performed in an analyzer for detecting the presence of a target nucleic acid in a sample. The process includes separating the target nucleic acid from other material present in the sample, performing an amplification reaction to produce an amplification product indicative of the presence of the target nucleic acid in the sample, and detecting the amplification product. The amplification reaction can be performed in an open receptacle vessel, such as a test tube.
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16 Claims
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1. A process for detecting the presence of a target nucleic acid in a sample containing the target nucleic acid, the process comprising performing the automated steps of:
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(a) immobilizing the target nucleic acid on a solid support comprising a magnetically-responsive particle; (b) subjecting the solid support to a magnetic field and aspirating non-immobilized components of the sample; (c) performing an amplification reaction using the target nucleic acid immobilized in step (a) to produce an amplification product indicative of the presence of the target nucleic acid in the sample; and (d) detecting the amplification product of step (c) as an indication of the presence of the target nucleic acid in the sample, wherein the steps of the process are performed in a self-contained analyzer. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A process for detecting the presence of a target nucleic acid in a sample containing the target nucleic acid, the process comprising performing the automated steps of:
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(a) immobilizing the target nucleic acid on a solid support comprising a magnetically-responsive particle; (b) subjecting the solid support to a magnetic field and aspirating non-immobilized components of the sample; (c) moving the target nucleic acid immobilized in step (a) into a temperature-controlled chamber of an incubator housed within a self contained analyzer; (d) in the chamber, performing an amplification reaction to produce an amplification product indicative of the presence of the target nucleic acid in the sample; and (e) detecting the amplification product of step (d) as an indication of the presence of the target nucleic acid in the sample, wherein the steps of the process are performed in the self-contained analyzer. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16)
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Specification