Detection of HPV
First Claim
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1. A kit comprising oligonucleotide detection assay components for detecting HPV sequences, wherein said components consist of:
- a) first, second and third pools of oligonucleotide sets, said sets consisting of first and second oligonucleotides, wherein the combination of first and second oligonucleotides form an invasive cleavage structure in combination with a target sequence comprising a HPV sequence, wherein said first pool comprises a first oligonucleotide of SEQ ID NO. 77 and a second oligonucleotide of SEQ ID NO. 78;
said second pool comprises a first oligonucleotide of SEQ ID NO. 127 and a second oligonucleotide of SEQ ID NO. 129; and
wherein said third pool comprises a first oligonucleotide of SEQ ID NO. 160 and a second oligonucleotide of SEQ ID NO. 161;
b) a FEN-1 endonuclease;
c) an internal control;
d) a plurality of fluorescence resonance energy transfer (FRET) cassettes; and
e) assay reagents comprising a buffer solution.
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Abstract
The present invention provides compositions and methods for the detection and characterization of HPV sequences. More particularly, the present invention provides compositions, methods and kits for using invasive cleavage structure assays (e.g. the INVADER assay) to screen nucleic acid samples, e.g., from patients, for the presence of any one of a collection of HPV sequences. The present invention also provides compositions, methods and kits for screening sets of HPV sequences in a single reaction container.
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10 Claims
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1. A kit comprising oligonucleotide detection assay components for detecting HPV sequences, wherein said components consist of:
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a) first, second and third pools of oligonucleotide sets, said sets consisting of first and second oligonucleotides, wherein the combination of first and second oligonucleotides form an invasive cleavage structure in combination with a target sequence comprising a HPV sequence, wherein said first pool comprises a first oligonucleotide of SEQ ID NO. 77 and a second oligonucleotide of SEQ ID NO. 78;
said second pool comprises a first oligonucleotide of SEQ ID NO. 127 and a second oligonucleotide of SEQ ID NO. 129; and
wherein said third pool comprises a first oligonucleotide of SEQ ID NO. 160 and a second oligonucleotide of SEQ ID NO. 161;b) a FEN-1 endonuclease; c) an internal control; d) a plurality of fluorescence resonance energy transfer (FRET) cassettes; and e) assay reagents comprising a buffer solution. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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Specification