Self-assembling peptide amphiphiles and related methods for growth factor delivery

• US 7,544,661 B2
• Filed: 12/06/2004
• Issued: 06/09/2009
• Est. Priority Date: 12/05/2003
• Status: Active Grant

• Current Assignee: Northwestern University
• Original Assignee: Northwestern University
• Inventors: Silva, Gabriel A., Behanna, Heather A., Donners, Jack J. J. M., Stupp, Samuel I., Anthony, Shawn G.
• Primary Examiner(s): Tsang; Cecilia
• Assistant Examiner(s): Cordero Garcia; Marcela M
• Application Number:
  US11/005,552
• Publication Number:
  US 20050209145A1
• Time in Patent Office: 1,646 Days
• Field of Search: None
• US Class Current: 514/1.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61P 19/00 Drugs for skeletal disorders
• CPC Class Codes: A61P 19/02 for joint disorders, e.g. arthritis, arthrosis
• CPC Class Codes: A61P 19/08 for bone diseases, e.g. rachitism, Paget's disease
• CPC Class Codes: A61P 25/00 Drugs for disorders of the nervous system
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 41/00 Drugs used in surgical methods, e.g. surgery adjuvants for preventing adhesion or for vitreum substitution
• CPC Class Codes: A61P 43/00 Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
• CPC Class Codes: C07K 14/475 Growth factors; Growth regulators
• CPC Class Codes: C07K 7/06 having 5 to 11 amino acids
• CPC Class Codes: C07K 7/08 having 12 to 20 amino acids gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68

Abstract

Amphiphilic peptide compounds comprising one or more epitope sequences for binding interaction with one or more corresponding growth factors, micellar assemblies of such compounds and related methods of use.

Claims

1. A method of using a micellar assembly to non-covalently interact with a growth factor to deliver and release said growth factor to a stem cell, said method comprising:

providing a growth factor and a micellar assembly comprising a plurality of a first amphiphilic compound selected from the group consisting of (SEQ ID NO;

     67);

2. The method of claim 1 wherein said growth factor is endogenously produced by said stem cell.

3. The method of claim 1 wherein said contact is sufficient for stem cell differentiation.

4. The method of claim 3 wherein said micellar assembly comprises the growth factor bone morphogenetic protein-2 (BMP-2).

5. The method of claim 4 wherein said stem cell is a mesenchymal stem cell.

6. The method of claim 5 wherein said stem cell differentiates into bone cells.

7. The method of claim 1 wherein said first amphiphilic compound is (SEQ ID NO:

67);

8. The method of claim 1 wherein said first amphiphilic compound is (SEQ ID NO:

68)

9. The method of claim 1 wherein said second amphiphilic peptide compound is (SEQ ID NO:

4);

10. The method of claim 1 wherein said second amphiphilic peptide compound is (SEQ ID NO:

69)