Automated process for detecting the presence of a target nucleic acid in a sample
DCFirst Claim
1. A process for detecting the presence of a target nucleic acid in a sample, the process comprising the automated steps of:
- (a) contacting a sample containing a target nucleic acid with a solid support comprising a magnetically-responsive particle;
(b) immobilizing the target nucleic acid on the solid support;
(c) at a first station of an analyzer, subjecting the solid support to a magnetic field and separating the immobilized target nucleic acid from a non-immobilized component of the sample;
(d) moving a solution comprising the separated target nucleic acid to a second station of the analyzer;
(e) in an incubator of the second station, subjecting the separated target nucleic acid to an amplification procedure and forming an amplification product; and
(f) detecting the amplification product formed in step (e) as an indication of the presence of the target nucleic acid in the sample.
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Abstract
An automated process for detecting the presence of a target nucleic acid in a sample. The process includes contacting a sample with a magnetically-responsive solid support and immobilizing a target nucleic acid present in the sample on the solid support. At a first station of an analyzer, the solid support is subjected to a magnetic field and the immobilized target nucleic acid is separated from a non-immobilized component of the sample. A solution comprising the separated target nucleic acid is moved to an incubator of a second station of the analyzer, where the separated target nucleic acid is subjected to an amplification procedure and an amplification product is formed. The amplification product is detected as an indication of the presence of the target nucleic acid in the sample.
363 Citations
36 Claims
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1. A process for detecting the presence of a target nucleic acid in a sample, the process comprising the automated steps of:
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(a) contacting a sample containing a target nucleic acid with a solid support comprising a magnetically-responsive particle; (b) immobilizing the target nucleic acid on the solid support; (c) at a first station of an analyzer, subjecting the solid support to a magnetic field and separating the immobilized target nucleic acid from a non-immobilized component of the sample; (d) moving a solution comprising the separated target nucleic acid to a second station of the analyzer; (e) in an incubator of the second station, subjecting the separated target nucleic acid to an amplification procedure and forming an amplification product; and (f) detecting the amplification product formed in step (e) as an indication of the presence of the target nucleic acid in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A process for detecting the presence of a target nucleic acid in a sample, the process comprising the automated steps of:
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(a) contacting a sample containing a target nucleic acid with a solid support comprising a magnetically-responsive particle; (b) immobilizing the target nucleic acid on the solid support; (c) at a first station of an analyzer, subjecting the solid support to a magnetic field and separating the immobilized target nucleic acid from a non-immobilized component of the sample; (d) forming a solution comprising the separated target nucleic acid and an amplification reagent in a reaction receptacle; (e) moving the reaction receptacle to a second station of the analyzer; (f) in an incubator of the second station, subjecting the separated target nucleic acid to an amplification procedure and forming an amplification product; and (g) detecting the amplification product formed in step (f) as an indication of the presence of the target nucleic acid in the sample. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36)
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Specification