NMR device for detection of analytes
First Claim
Patent Images
1. A device for detection of an analyte, the device comprising:
- a support defining a well for holding a liquid sample comprising magnetic particles and said analyte, said magnetic particles having binding moieties linked thereto; and
an RF coil disposed about said liquid sample, said RF coil configured to detect an echo response produced by exposing said liquid sample to a bias magnetic field created using one or more magnets and an RF excitation, wherein said RF coil has a characteristic dimension from about 10 μ
m to about 1000 μ
m;
wherein said magnetic particles are deposited onto a surface of said well and wherein said magnetic particles are reconstitutable upon introduction of liquid.
2 Assignments
0 Petitions
Accused Products
Abstract
This invention relates generally to detection devices having one or more small wells each surrounded by, or in close proximity to, an NMR micro coil, each well containing a liquid sample with magnetic nanoparticles that self-assemble or disperse in the presence of a target analyte, thereby altering the measured NMR properties of the liquid sample. The device may be used, for example, as a portable unit for point of care diagnosis and/or field use, or the device may be implanted for continuous or intermittent monitoring of one or more biological species of interest in a patient.
146 Citations
49 Claims
-
1. A device for detection of an analyte, the device comprising:
-
a support defining a well for holding a liquid sample comprising magnetic particles and said analyte, said magnetic particles having binding moieties linked thereto; and an RF coil disposed about said liquid sample, said RF coil configured to detect an echo response produced by exposing said liquid sample to a bias magnetic field created using one or more magnets and an RF excitation, wherein said RF coil has a characteristic dimension from about 10 μ
m to about 1000 μ
m;wherein said magnetic particles are deposited onto a surface of said well and wherein said magnetic particles are reconstitutable upon introduction of liquid. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
-
-
29. A device for detection of an analyte, said device comprising a plurality of wells for holding a liquid sample comprising magnetic particles and said analyte, said magnetic particles having binding moieties linked thereto, and, for each of said wells:
-
an RF coil disposed about said well, said RF coil configured to detect an echo response produced by exposing said liquid sample in said well to a bias magnetic field created using one or more magnets and an RF excitation; wherein at least one well has a known characteristic substantially unaffected by said analyte and is used for calibration. - View Dependent Claims (30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43)
-
-
44. A device comprising:
-
a support defining one or more wells for holding a liquid sample; and disposed on said support, for reconstitution within said one or more wells, dried superparamagnetic particles having binding moieties linked thereto, wherein said binding moieties are operative to alter an aggregation of said magnetic particles in said liquid sample as a function of the presence or concentration of an analyte in said liquid sample. - View Dependent Claims (45)
-
-
46. A device for detection of an analyte, the device comprising:
-
a support defining a well for holding a liquid sample comprising magnetic particles and said analyte, said magnetic particles having binding moieties linked thereto; and an RF coil disposed about said liquid sample, said RF coil configured to detect an echo response produced by exposing said liquid sample to a bias magnetic field created using one or more magnets and an RF excitation, wherein said RF coil has a characteristic dimension from about 10 μ
m to about 1000 μ
m;wherein said device is implantable, and is operable without skin-penetrating wires.
-
-
47. A device for detection of an analyte, said device comprising a plurality of wells for holding a liquid sample comprising magnetic particles and said analyte, said magnetic particles having binding moieties linked thereto, and, for each of said wells:
-
an RF coil disposed about said well, said RF coil configured to detect an echo response produced by exposing said liquid sample in said well to a bias magnetic field created using one or more magnets and an RF excitation; wherein magnetic particles having different binding moieties are disposed in different wells for detection of said analyte, said different binding moieties promoting aggregation or disaggregation of said magnetic particles in proportion to concentration of said analyte.
-
-
48. A device for detection of an analyte, said device comprising a plurality of wells for holding a liquid sample comprising magnetic particles and said analyte, said magnetic particles having binding moieties linked thereto, and, for each of said wells:
-
an RF coil disposed about said well, said RF coil configured to detect an echo response produced by exposing said liquid sample in said well to a bias magnetic field created using one or more magnets and an RF excitation; wherein said magnetic particles are deposited onto a surface of one or more of said plurality of wells. - View Dependent Claims (49)
-
Specification