Detection of analytes using reorganization energy

• US 7,579,145 B2
• Filed: 10/30/2007
• Issued: 08/25/2009
• Est. Priority Date: 06/12/1997
• Status: Expired due to Fees

• Current Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Original Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Inventors: Meade, Thomas J.
• Primary Examiner(s): Monshipouri; Maryam
• Application Number:
  US11/978,971
• Publication Number:
  US 20080173553A1
• Time in Patent Office: 665 Days
• Field of Search: 435 5- 7, 435/7.71, 435/7.8, 435/7.9, 435/7.91, 205/793.5, 205/777.5, 205/778
• US Class Current: 435/5
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: G01N 33/542 with steric inhibition or signal modification, e.g. fluorescent quenching
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

The invention relates to novel methods and compositions for the detection of analytes using the nuclear reorganization energy, λ, of an electron transfer process.

Claims

1. A method of detecting a target analyte in a test sample, said method comprising:

(1) adding a target analyte to a composition comprising an electrode, said electrode comprising;

(a) a redox active complex comprising;

(i) a redox active molecule, wherein said redox active molecule is a transition metal selected from the group consisting of manganese, technetium, rhenium, iron, ruthenium, osmium, cobalt, rhodium, iridium, nickel, palladium, platinum, copper, silver and gold; and

(ii) a binding ligand, wherein at least one atom of the binding ligand serves as a coordination moiety to said transition metal to form said redox active complex, and wherein said redox active complex is attached to said electrode via a spacer; and

(b) a self-assembled monolayer; and

(2) detecting said target analyte.

2. The method according to claim 1, wherein said metal is osmium.

3. The method according to claim 1, wherein said attachment of said redox active molecule and said binding ligand have a conformation of:

4. The method according to claim 1, wherein said redox active molecule comprises a cyano group.

5. The method according to claim 1, wherein said binding ligand is a protein.

6. The method according to claim 5, wherein said protein is a peptide.

7. The method according to claim 1, wherein said target analyte is an enzyme and said binding ligand is a substrate of said enzyme.

8. The method according to claim 1, wherein said spacer is an insulator.

9. The method according to claim 1, wherein said spacer is conductive oligomer.

10. The method according to claim 1, wherein said detecting step is carried out using voltammetry.

11. A method of detecting a target analyte in a test sample, said method comprising:

(1) adding a target analyte to the composition comprising an electrode, said electrode comprising;

(a) a redox active complex comprising;

(i) a redox active molecule, said redox active molecule comprising osmium and at least one cyano group; and

(ii) a binding ligand comprising a peptide, wherein at least one atom of the binding ligand serves as a coordination moiety to said osmium to form said redox active complex, and wherein said redox active complex is attached to said electrode via a spacer; and

(b) a self-assembled monolayer; and

(2) detecting said target analyte.