Method for producing a colorimetric resonant reflection biosensor on rigid surfaces

• US 7,615,339 B2
• Filed: 07/13/2006
• Issued: 11/10/2009
• Est. Priority Date: 10/30/2000
• Status: Expired due to Fees

• Current Assignee: X-Body Incorporated (Bristol-Myers Squibb Company)
• Original Assignee: Sru Biosystems Incorporated
• Inventors: Cunningham, Brian T., Qiu, Jean
• Primary Examiner(s): Chin; Christopher L
• Application Number:
  US11/485,867
• Publication Number:
  US 20060286663A1
• Time in Patent Office: 1,216 Days
• Field of Search: None
• US Class Current: 435/4
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/4788 Diffraction for sizing particles G01N15/0205
• CPC Class Codes: G01N 33/54373 involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
• CPC Class Codes: G02B 5/1809 with pitch less than or comparable to the wavelength
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus

Abstract

The invention provides methods for fabricating calorimetric resonant reflection biosensors.

Claims

1. A method for casting a colorimetric resonant reflection biosensor structure comprising:

(a) applying a liquid or semi-solid material that is capable of being transformed or cured into a flexible master structure over a rigid colorimetric resonant reflection biosensor master structure;

(b) transforming or curing the liquid or semi-solid material into a flexible master structure, wherein the flexible master structure has the rigid colorimetric resonant reflection biosensor master structure embossed into a first surface of the flexible master structure; and

(c) peeling the flexible master structure from the rigid colorimetric resonant reflection biosensor master structure;

(d) pressing the flexible master structure into a liquid or semi-solid layer, wherein the liquid or semi-solid layer is on a rigid substrate; and

(e) transforming or curing the liquid or semi-solid layer into a solid layer;

whereby a colorimetric resonant reflection biosensor structure is casted.

2. The method of claim 1, wherein the rigid colorimetric resonant reflection biosensor master structure is etched into a silicon or glass wafer surface.

3. The method of claim 1, wherein the flexible master structure comprises a grating pattern selected from the group consisting of squares, triangles, sinusoidal waves, inverted “

u”

shapes, lines, circles, ellipses, trapezoids, ovals, rectangles, hexagons, phase-quantized terraced surface relief patterns whose groove pattern resembles a stepped pyramid, and concentric rings.

4. The method of claim 1, wherein the flexible master structure comprises a grating pattern having a periodic spacing of between about 0.1 microns to about 2.0 microns.

5. The method of claim 1, wherein the flexible master structure comprises a submicron grating pattern.

6. The method of claim 5, wherein the grating pattern has a periodic spacing of between about 0.2 microns to about 0.6 microns.

7. The method of claim 1, further comprising removing the flexible master structure from the solid layer.

8. The method of claim 7, further comprising depositing a high refractive index dielectric film over the solid layer.

9. The method of claim 8, further comprising immobilizing one or more specific binding substances on the high refractive index dielectric film.

10. The method of claim 1, wherein an adhesion-enhancing film is applied to the rigid colorimetric resonant reflection biosensor master structure before the liquid or semi-solid material that is capable of being transformed or cured into a flexible master structure is applied over the rigid colorimetric resonant reflection biosensor master structure.