Real-time PCR in micro-channels

  • US 7,629,124 B2
  • Filed: 08/17/2006
  • Issued: 12/08/2009
  • Est. Priority Date: 06/30/2006
  • Status: Active Grant
First Claim
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1. A method of performing real-time PCR comprising the steps of:

  • a) continuously moving a bolus of test solution containing real-time PCR reagents in a channel;

    b) moving a carrier fluid in said channel, sequentially alternating with a test bolus;

    c) cycling the temperature in a defined section of the channel in order to achieve PCR, wherein said defined section of the channel has a uniform temperature, which temperature cycles in time; and

    d) measuring the intensity of the fluorescent signal at a plurality of locations along said defined section of said channel.

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