Method of determining the nucleotide sequence of oligonucleotides and DNA molecules

  • US 7,645,596 B2
  • Filed: 05/05/2004
  • Issued: 01/12/2010
  • Est. Priority Date: 05/01/1998
  • Status: Expired due to Term
First Claim
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1. A method for sequencing nucleic acid, the method comprising the steps of:

  • hybridizing nucleic acid to a complementary nucleic acid sequence to form one or more duplexes;

    adding at least one nucleotide comprising a detectable label to said duplex, wherein said nucleotide is not a dideoxy nucleotide and wherein said nucleotide has a free 3′

    hydroxyl when added to said duplex;

    identifying said nucleotide comprising said detectable label; and

    repeating said adding and identifying steps, thereby to determine the sequence of said nucleic acid.

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