IdeS, an IgG-degrading enzyme of Streptococcus pyogenes

  • US 7,666,582 B2
  • Filed: 12/17/2002
  • Issued: 02/23/2010
  • Est. Priority Date: 12/18/2001
  • Status: Active Grant
First Claim
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1. A method of generating Fc or Fab fragments of IgG comprising:

  • (i) contacting IgG with;

    a) polypeptide having the sequence of SEQ ID NO;

    1, orb) a polypeptide having the sequence of SEQ ID NO;

    1 except for 1, 2 or 3 conservative substitutions,wherein said polypeptide (a) and said polypeptide (b) have a cysteine residue at the position corresponding to position 65 of SEQ ID NO;

    1, position 65 of SEQ ID NO;

    1 corresponding to position 94 of SEQ ID NO;

    2,wherein IgG is contacted with said polypeptide (a) or said polypeptide (b) under conditions that permit the IgG specific cysteine protease activity of said polypeptide (a) or said polypeptide (b);

    (ii) thereby generating said fragments of IgG; and

    (iii) isolating the fragments of IgG obtained, or analysing the starting materials or reaction products to determine whether the desired product is present.

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