Escherichia bacteria transformed with the yddG gene to enhance L-amino acid producing activity

CAFC
  • US 7,666,655 B2
  • Filed: 11/25/2002
  • Issued: 02/23/2010
  • Est. Priority Date: 11/23/2001
  • Status: Active Grant
First Claim
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1. A recombinant Escherichia coli bacterium that has the ability to produce and accumulate an aromatic L-amino acid, wherein the aromatic L-amino acid production by said bacterium is enhanced by enhancing activity of a protein in a cell of said bacterium beyond the levels observed in a wild-type of said bacterium, wherein said protein is as defined in the following (A), (B), or (C):

  • (A) a protein which consists of the amino acid sequence of SEQ ID NO;

    2;

    (B) a protein which consists of the amino acid sequence of SEQ ID NO;

    2 except wherein one to five amino acids are deleted, substituted, inserted, or added;

    or(C) a protein which consists of the amino acid sequence that is encoded by a nucleotide sequence that hybridizes with the complement of the nucleotide sequence of SEQ ID NO;

    1 under stringent conditions comprising 60°

    C., 1×

    SSC, 0.1% SDS;

    wherein said protein defined in (A), (B), or (C) has an activity to make said bacterium resistant to L-phenylalanine, fluoro-phenylalanine or 5-fluoro-DL-tryptophan and wherein the activity of said protein defined in (A), (B), or (C) is enhanced by;

    a) transformation of said bacterium with a DNA encoding said protein and expressing the protein in said bacterium,b) replacing the native promoter that precedes the DNA encoding said protein on the chromosome of the bacterium with a more potent promoter, orc) introduction of multiple copies of the DNA encoding said protein into the chromosome of said bacterium and expressing the protein in said bacterium.

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