Microfluidic device and methods of using same

• US 7,691,333 B2
• Filed: 04/05/2004
• Issued: 04/06/2010
• Est. Priority Date: 11/30/2001
• Status: Active Grant

• Current Assignee: Standard Biotools Incorporated
• Original Assignee: Fluidigm Corporation
• Inventors: Yi, Yong, Unger, Marc, Facer, Geoffrey, Nassef, Hany Ramez, Lucero, Michael, McBride, Lincoln
• Primary Examiner(s): Warden; Jill
• Assistant Examiner(s): LEVKOVICH, NATALIA A
• Application Number:
  US10/819,088
• Publication Number:
  US 20050019792A1
• Time in Patent Office: 2,192 Days
• Field of Search: 422/100, 422/103, 422/102
• US Class Current: 422/503
• CPC Class Codes: B01F 33/30 Micromixers
• CPC Class Codes: B01J 2219/00337 Valves
• CPC Class Codes: B01J 2219/00353 Pumps
• CPC Class Codes: B01J 2219/00403 in multiple arrangements
• CPC Class Codes: B01J 2219/00495 Means for heating or cooling the reaction vessels
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/0059 Sequential processes
• CPC Class Codes: B01J 2219/0072 Organic compounds
• CPC Class Codes: B01L 2300/0627 Sensor or part of a sensor is integrated
• CPC Class Codes: B01L 2300/0883 Serpentine channels
• CPC Class Codes: B01L 2300/123 Flexible; Elastomeric
• CPC Class Codes: B01L 2400/06 Valves, specific forms thereof
• CPC Class Codes: B01L 3/00 Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: B01L 3/50851 specially adapted for heating or cooling samples laboratory heating apparatus B01L7/00; incubators C12M
• CPC Class Codes: C12Q 1/6844 Nucleic acid amplification reactions
• CPC Class Codes: C12Q 2565/501 being an array of oligonucleotides
• CPC Class Codes: C40B 60/08 Integrated apparatus specially adapted for both creating and screening libraries
• CPC Class Codes: C40B 60/14 : for creating libraries
• CPC Class Codes: G01N 2035/00366 : Several different temperatures used
• CPC Class Codes: G01N 35/00029 : provided with flat sample substrates, e.g. slides G01N35/028 takes precedence
• CPC Class Codes: G01N 35/0098 : involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: Y10T 436/25 : including sample preparation

Abstract

A variety of elastomeric-based microfluidic devices and methods for using and manufacturing such devices are provided. Certain of the devices have arrays of reaction sites to facilitate high throughput analyses. Some devices also include reaction sites located at the end of blind channels at which reagents have been previously deposited during manufacture. The reagents become suspended once sample is introduced into the reaction site. The devices can be utilized with a variety of heating devices and thus can be used in a variety of analyses requiring temperature control, including thermocycling applications such as nucleic acid amplification reactions, genotyping and gene expression analyses.

Claims

1. A microfluidic device comprising:

a first fluidic channel having a first end and a second end in fluid communication with each other through said channel;

a plurality of blind flow channels, each blind flow channel terminating at a terminal wall;

wherein each blind flow channel branches from and is in fluid communication with said first fluidic channel,

wherein an aqueous fluid that enters one of said blind flow channels from said first fluidic channel can flow out of the blind flow channel only through said first fluidic channel; and

,

an inlet in fluid communication with said first fluidic channel, said inlet for introducing a sample fluid;

wherein each blind flow channel is associated with a valve that when closed isolates one end of the blind flow channel from the first fluidic channel, whereby an isolated reaction site is formed between the valve and the terminal wall;

a control channel;

wherein each said valve is a deflectable membrane which is deflected into the blind flow channel associated with the valve when an actuating force is applied to the control channel, thereby closing the valve; and

wherein when the actuating force is applied to the control channel the valve in each of said blind flow channels is closed, so as to produce the isolated reaction site in each blind flow channel.

2. The microfluidic device of claim 1 wherein said fluidic channel and said blind flow channels are formed in an elastomeric substrate.

3. The microfluidic device of claim 1 wherein said reaction cells are formed within an elastomeric block formed from a plurality of layers bonded together and said valve is a deflectable membrane.

4. The microfluidic device of claim 1 wherein the fluid capacity of each isolated reaction site is less than 20 nL.

5. The microfluidic device of claim 1 wherein the fluid capacity of each isolated reaction site is less than 10 nL.

6. The microfluidic device of claim 1 wherein deflectable membrane is formed from an elastomeric material having a Young'"'"'s modulus in the range 50 Pa to 10 MPa.

7. The microfluidic device of claim 6 wherein deflectable membrane is formed from an elastomeric material having a Young'"'"'s modulus in the range 100 Pa to 1 MPa.

8. The microfluidic device of claim 7 wherein the deflectable membrane is formed from polydimethylsiloxane (PDMS).

9. The microfluidic device of claim 1 comprising an array of reaction sites having a density of at least 100 sites/cm².

10. The microfluidic device of claim 1 comprising an array of reaction sites having a density of at least 1000 sites/cm².

11. The device of claim 1 wherein each blind flow channel comprises a second valve that when closed forms a second isolated reaction site.

12. A method of analyzing a nucleic acid in a sample fluid for a reaction comprising the steps of:

(i) providing a microfluidic device, said microfluidic device comprising;

a first fluidic channel having a first end and a second end in fluid communication with each other through said channel;

a plurality of blind flow channels, each blind flow channel terminating at a terminal wall;

wherein each blind flow channel branches from and is in fluid communication with said first fluidic channel,

wherein an aqueous fluid that enters one of said blind flow channels from said first fluidic channel can flow out of the blind flow channel only through said first fluidic channel; and

,

an inlet in fluid communication with said first fluidic channel, said inlet for introducing a sample fluid;

wherein each blind flow channel is associated with a valve that when closed isolates one end of the blind flow channel from the first fluidic channel, whereby an isolated reaction site is formed between the valve and the terminal wall;

a control channel;

wherein each said valve is a deflectable membrane which is deflected into the blind flow channel associated with the valve when an actuating force is applied to the control channel, thereby closing the valve; and

wherein when the actuating force is applied to the control channel a valve in each of said blind flow channels is closed, so as to produce the isolated reaction site in each blind flow channel;

(ii) introducing said sample fluid into said inlet, said sample fluid filling said blind flow channels;

(iii) actuating said valve to separate said sample fluid into said separate portions within said blind flow channels;

(iv) amplifying the nucleic acid in said sample fluid;

(v) analyzing said portions of said sample fluid to determine whether said amplifying produced said reaction.

13. The method of claim 12, wherein said sample fluid comprises an amplifiable nucleic acid target and said conditions are polymerase chain reaction (PCR) conditions, and said reaction results in a PCR product being formed.

14. The method of claim 13, wherein said PCR is digital PCR.

15. The method of claim 13, wherein said reaction produces two different color results.

16. The method of claim 12 wherein the analysis is single nucleotide polymorphism analysis.

17. The method of claim 12 wherein the analysis is genotyping.

18. The method of claim 12 wherein the analysis is real time PCR.

19. The method of claim 12 wherein each reaction volume is less than 20 nL.

20. The method of claim 12 wherein each reaction volume is less than 10 nL.

21. The method of claim 12 wherein said sample is partitioned into at least 500 isolated reaction volumes.

22. The method of claim 12 wherein each said blind flow channel comprises a second valve that when closed forms a second isolated reaction site.

23. The method of claim 12 wherein said valves are formed from an elastomeric membrane that when actuated, deflects into said blind flow channel.

24. The method of claim 23 wherein the membrane is formed from an elastomeric material having a Young'"'"'s modulus in the range 50 Pa to 10 MPa.

25. The method of claim 23 wherein the membrane is formed from an elastomeric material having a Young'"'"'s modulus in the range 100 Pa to 1 MPa.

26. The method of claim 23 wherein the membrane is formed from polydimethylsiloxane (PDMS).

27. The method of claim 12 wherein the amplification is achieved by thermocycling.