Implantable system for glucose monitoring using fluorescence quenching
First Claim
1. A system for measuring glucose, said system comprising:
- (a) an implantable glucose-sensing device comprising;
a hydrogel having a matrix;
a functionalized polymer or functionalized nano-particle ligand;
a sugar-binding molecule comprising Concanavalin A, other sugar-binding lectins, glucose binding protein, glucose oxidase, or glucose apo-oxidase; and
a first fluorophore comprising a fluorescein, a rhodamine, a cyanine, or a fluorescent dye linked to the ligand or the sugar-binding molecule;
wherein the ligand and the sugar-binding molecule are present throughout the matrix of the hydrogel,wherein the first fluorophore is a quenchable fluorophore having a fluorescent emission spectrum operable to be quenched upon binding or release of glucose by the sugar-binding molecule; and
(b) a light source configured and arranged to illuminate the implantable glucose sensing device with light of a wavelength sufficient to excite the quenchable fluorophore;
(c) a detector (a) in optical communication with the implantable glucose sensing device and (b) configured and arranged to detect at least at least one wavelength of light from the implantable glucose sensing device and produce a detected light signal;
(d) a processor in communication with the detector and configured and arranged to receive and process at least one detected light signal to produce a glucose metric;
(e) a display operably linked to the processor and configured and arranged to receive and display the glucose measurement; and
(f) a power source operably linked to at least the light source, the detector, the processor, and the display.
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Accused Products
Abstract
This disclosure relates to systems, devices, and methods of sensing an analyte. An implantable sensor may be contacted with a test sample under conditions that permit a binding protein and a ligand of the sensor to interact in an analyte-dependent manner to produce an analyte-dependent signal, and (b) detecting the analyte-dependent signal with a detector. A binding protein may reversibly bind an analyte and/or a ligand. A binding protein may have a higher binding affinity for an analyte than for a ligand. A binding protein and a ligand may each include a fluorophore, the absorption and/or emission properties of which may change in an analyte-dependent manner. A binding protein and/or a ligand may be bound to an active or inactive substrate. Some embodiments of systems, devices, and methods may be practiced in vitro, in situ, and/or in vivo. Systems and/or devices of the disclosure may be configured to be wearable.
26 Citations
22 Claims
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1. A system for measuring glucose, said system comprising:
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(a) an implantable glucose-sensing device comprising; a hydrogel having a matrix; a functionalized polymer or functionalized nano-particle ligand; a sugar-binding molecule comprising Concanavalin A, other sugar-binding lectins, glucose binding protein, glucose oxidase, or glucose apo-oxidase; and a first fluorophore comprising a fluorescein, a rhodamine, a cyanine, or a fluorescent dye linked to the ligand or the sugar-binding molecule; wherein the ligand and the sugar-binding molecule are present throughout the matrix of the hydrogel, wherein the first fluorophore is a quenchable fluorophore having a fluorescent emission spectrum operable to be quenched upon binding or release of glucose by the sugar-binding molecule; and (b) a light source configured and arranged to illuminate the implantable glucose sensing device with light of a wavelength sufficient to excite the quenchable fluorophore; (c) a detector (a) in optical communication with the implantable glucose sensing device and (b) configured and arranged to detect at least at least one wavelength of light from the implantable glucose sensing device and produce a detected light signal; (d) a processor in communication with the detector and configured and arranged to receive and process at least one detected light signal to produce a glucose metric; (e) a display operably linked to the processor and configured and arranged to receive and display the glucose measurement; and (f) a power source operably linked to at least the light source, the detector, the processor, and the display. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification