Biosensor with inorganic-organic hybrid polymer coating

• US 7,709,221 B2
• Filed: 11/14/2006
• Issued: 05/04/2010
• Est. Priority Date: 11/17/2005
• Status: Expired due to Fees

• Current Assignee: École centrale de Lyon, Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V., Institute of Catalysis
• Original Assignee: École centrale de Lyon, Fraunhofer Gesellschaft Zur Foerderung Der Angewandten Forsching E.V., Institute of Catalysis
• Inventors: Betancor, Lorena, Lafuente, Roberto Fernandez, Rose, Klaus, Guisan, Jose Manuel, Jaffrezic, Nicole, Dzyadevych, Sergei
• Primary Examiner(s): GITOMER, RALPH J
• Application Number:
  US11/598,935
• Publication Number:
  US 20070161069A1
• Time in Patent Office: 1,267 Days
• Field of Search: 435/14, 435/287.9, 435/817, 204/403.11
• US Class Current: 435/14
• CPC Class Codes: C12Q 1/001 Enzyme electrodes
• CPC Class Codes: C12Q 1/006 for glucose
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

The present invention relates to a biosensor comprising a substrate with a coating system in which a Ruthenium complex and an enzyme is integrated. The enzyme is able to convert bioproducts, e.g. glucose, fructose or glycerol. The depletion of oxygen during these converting reactions can be monitored via the fluorescence of the Ruthenium complex. The inventive biosensor can be used in biotechnological processes, e.g. the synthesis of biofuels.

Claims

1. A biosensor comprising a substrate with at least one coating of an inorganic-organic hybrid polymer as a matrix for at least one Ruthenium complex selected from the group consisting of Ruthenium tris-(2,2′

-bipyridyl) dichloride (II);

2. The biosensor of claim 1, wherein the substrate is a glass and/or a polymer.

3. The biosensor of claim 1, wherein the substrate is an optical lens or an optical fibre.

4. The biosensor of claim 1, wherein the substrate is an optical fibre having a cladding layer.

5. The biosensor of claim 1, wherein the enzyme is selected from the group consisting of glucose oxidase from Penicillinium vitale, glucose oxidase from Aspergillus niger, glucose oxidase from Aspergillus niger type II and glucose oxidase from Aspergillus niger type IIS.

6. The biosensor of claim 1, wherein the enzyme is selected from the group consisting of glucose oxidase, glucose isomerase and glucose catalase.

7. The biosensor of claim 1, wherein the enzyme is selected from the group consisting of glycerokinase, L-Glycerol-3-phosphate and catalase.

8. The bio sensor of claim 1, wherein the enzyme is a stabilized enzyme.

9. The biosensor of claim 8, wherein the stabilized enzyme is an enzyme stabilized by immobilization in a polymeric host matrix.

10. The biosensor of claim 9, wherein the polymeric host matrix is selected from the group polystyrene, styrene/divinylbenzene copolymer and polyacrylics.

11. The biosensor of claim 1, wherein the alkoxy compound of a metal is selected from the group consisting of Si(OEt)₄, Ti(OEt)₄, Zr(OPr)₄ and Al(O^sBu)₃.

12. The biosensor of claim 1, wherein the substrate has a single coating comprising the at least one Ruthenium complex and the at least one enzyme.

13. The biosensor of claim 1, wherein the substrate has a first coating containing the at least one Ruthenium complex and a second coating containing the at least one enzyme.

14. The biosensor of claim 1, wherein the additional organic network is formed by the polymerization of monomers having reactive groups that are UV-curable.

15. The biosensor of claim 14, wherein the monomers are selected from the group consisting of glycidoxypropyltrimethoxysilane, vinyltrimethoxysilane, methacryloxypropyltrimethoxysilane, methacryloxypropylmethyl dimethoxysilane, acryloxypropylmethyl dimethoxysilane, N-(3-acryloxy-2-hydroxypropyl)-3-aminopropyltri-ethoxysilane, tripropyleneglycolacrylate trimethoxysilane, triethyleneglycolacrylate trimethoxysilane, tetraethyleneglycolacrylate trimethoxysilane, bisphenol-A-acrylate methyldimethoxysilane, glycerindimethacrylate triethoxysilane-a, glycerindimethacrylate triethoxysilane-b, butanediol-diglycidylether-diacrylate triethoxysilane-a, butanediol-diglycidylether-diacrylate triethoxysilane-b, propoxylated glyceryldiacrylate trimethoyxysilane, trimethylopropanediacrylate methyldimethoyxysilane, ethoxylated trimethylolpropane diacrylate trimethoxysilane, pentaerythritoltriacrylate triethoxysilane-a, and pentaerythritoltriacrylate triethoxysilane-b.