Immunoassay device with immuno-reference electrode

• US 7,723,099 B2
• Filed: 09/10/2003
• Issued: 05/25/2010
• Est. Priority Date: 09/10/2003
• Status: Active Grant

• Current Assignee: Abbott Point Of Care Incorporated (Abbott Laboratories Incorporated)
• Original Assignee: Abbott Point Of Care Incorporated (Abbott Laboratories Incorporated)
• Inventors: Miller, Cary James, Campbell, John Lewis Emerson
• Primary Examiner(s): Yu; Melanie J.
• Application Number:
  US10/658,529
• Publication Number:
  US 20060160164A1
• Time in Patent Office: 2,449 Days
• Field of Search: 422/50, 422/55, 422/57, 422/58, 422/61, 422/68.1, 422/82.05, 422/82.01, 435/4, 435/7.1, 435/7.7, 435/283.1, 435/287.1, 435/288.7, 436/518, 436/524, 436/525, 436/528
• US Class Current: 435/287.1
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10T 436/25 including sample preparation

Abstract

An electrochemical immunosensor system with reduced interference, comprising: a first immunosensor that generates an electrochemical signal based on the formation of a sandwich between an immobilized antibody, a target analyte and a labeled antibody, wherein a portion of the signal arises from non-specific binding of the labeled antibody in the region of the first immunosensor, and

• • a second immunosensor that acts as an immuno-reference sensor and generates a signal that is the same as or predictably related to the degree of non-specific binding which occurs in the region of the first immunosensor, and has an immunocomplex between an immobilized antibody and an endogenous or exogenous protein that is in the sample and that is not the target analyte.

Claims

1. An immunosensor system with reduced interference, comprising:

a first immunosensor that includes a first immobilized antibody and generates a first signal based on a sandwich between the first immobilized antibody, a target analyte and a labeled antibody, wherein a portion of the first signal arises from a degree of non-specific binding of the labeled antibody, and

a second immunosensor that includes a second immobilized antibody and acts as an immuno-reference sensor and generates a second signal that is the same as or predictably related to the degree of non-specific binding, and has an immunocomplex between the second immobilized antibody and an endogenous or exogenous protein that is in a sample and that is not the target analyte; and

a subtraction analyzer configured to determine a corrected signal from the first and second signals.

2. The immunosensor system of claim 1, wherein the first and second immunosensors are electrochemical sensors.

3. The immunosensor system of claim 1, wherein the first immunosensor and the second immunosensor are in a disposable cartridge for measuring analytes in the sample.

4. The immunosensor system of claim 1, wherein the target analyte is selected from the group consisting of troponin I, troponin T, creatine kinase MB, procalcitonin, hCG, NTproBNP, proBNP, BNP and myoglobin, in the sample.

5. The immunosensor system of claim 3, wherein the second immobilized antibody is to a plasma protein.

6. The immunosensor system of claim 3, wherein the endogenous or exogenous protein in the sample is present at a concentration sufficient to bind more than 50% of the available second immobilized antibody within about 100 seconds of the sample contacting the immunosensor system.

7. The immunosensor system of claim 3, wherein the second immobilized antibody has an affinity constant of about 1×

10(−

7) to 1×

10(−

15).

8. The immunosensor system of claim 1, wherein both of the first and second immobilized antibodies are immobilized on microparticles of diameter in the range 0.01-5.0 μ

m.

9. The immunosensor system of claim 1, wherein the endogenous or exogenous protein is present in the sample at a concentration of at least three orders of magnitude above the affinity constant of the second immobilized antibody.

10. The immunosensor system of claim 4, wherein the sample comprises a blood sample.

11. The immunosensor system of claim 9, wherein the sample comprises a blood sample.

12. The immunosensor system of claim 1, wherein the non-specific binding occurs on the first immunosensor.