Tumor suppressor designated TS10Q23.3

• US 7,732,576 B2
• Filed: 10/30/2006
• Issued: 06/08/2010
• Est. Priority Date: 01/30/1997
• Status: Expired due to Fees

• Current Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Original Assignee: Board of Regents of the University of Texas System (University of Texas System)
• Inventors: Tavtigian, Sean V., Yung, Alfred W. K., Jasser, Samar A., Steck, Peter, Pershouse, Mark A.
• Primary Examiner(s): Rawlings; Stephen L
• Assistant Examiner(s): DUFFY, BRADLEY
• Application Number:
  US11/590,556
• Publication Number:
  US 20070054333A1
• Time in Patent Office: 1,317 Days
• Field of Search: 530/387.1, 530/387.3, 530/350, 530/87.1, 530/387.9, 530/388.1
• US Class Current: 530/387.1
• CPC Class Codes: A01K 2217/075 inducing loss of function, i.e. knock out
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 48/00 Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
• CPC Class Codes: C07K 14/4703 Inhibitors; Suppressors
• CPC Class Codes: C12N 2799/021 for the expression of a heterologous nucleic acid
• CPC Class Codes: C12N 9/16 acting on ester bonds (3.1)
• CPC Class Codes: C12Q 1/6886 for cancer immunoassay for cancer G01N33/574
• CPC Class Codes: C12Q 2600/112 Disease subtyping, staging or classification
• CPC Class Codes: C12Q 2600/136 Screening for pharmacological compounds
• CPC Class Codes: C12Q 2600/16 Primer sets for multiplex assays
• CPC Class Codes: C12Q 2600/172 Haplotypes
• CPC Class Codes: G01N 2333/916 acting on ester bonds (3.1), e.g. phosphatases (3.1.3), phospholipases C or phospholipases D (3.1.4)
• CPC Class Codes: G01N 33/57407 Specifically defined cancers
• CPC Class Codes: G01N 33/57434 of prostate
• CPC Class Codes: G01N 33/57496 involving intracellular compounds

Abstract

A specific region of chromosome 10 (10q23.3) has been implicated by series of studies to contain a tumor suppressor gene involved in gliomas, as well as a number of other human cancers. One gene within this region was identified, and the corresponding coding region of the gene represents a novel 47 kD protein. A domain of this product has an exact match to the conserved catalytic domain of protein tyrosine phosphatases, indicating a possible functional role in phosphorylation events. Sequence analyses demonstrated the a number of exons of the gene were deleted in tumor cell lines used to define the 10q23.3 region, leading to the classification of this gene as a tumor suppressor. Further analyses have demonstrated the presence of a number of mutations in the gene in both glioma and prostate carcinoma cells. Methods for diagnosing and treating cancers related to this tumor suppressor, designated as TS10q23.3, also are disclosed.

Claims

1. An isolated antibody or antibody fragment that binds specifically to a protein comprising the amino acid sequence encoded by the coding sequence comprising positions 1035 to 2243 of the nucleotide sequence of SEQ ID NO:

1, wherein said antibody or antibody fragment does not specifically bind an epitope consisting of phosphotyrosine or phosphoserine.

2. The isolated antibody of claim 1, wherein said antibody is a polyclonal antibody, a monoclonal antibody or a humanized antibody.

3. The isolated antibody of claim 2 wherein said antibody is a monoclonal antibody.

4. A kit comprising an isolated antibody or antibody fragment that binds specifically to a protein comprising the amino acid sequence encoded by the coding sequence comprising positions 1035 to 2243 of the nucleotide sequence of SEQ ID NO:

1, wherein said antibody or antibody fragment does not specifically bind an epitope consisting of phosphotyrosine or phosphoserine.

5. An isolated antibody or antibody fragment that binds specifically to a protein comprising the amino acid sequence encoded by the coding sequence comprising positions 1035 to 2243 of the nucleotide sequence of SEQ ID NO:

1, wherein said antibody or antibody fragment is non-cross reactive with other human proteins.