Cells in which activity of the protein involved in transportation of GDP-fucose is reduced or lost

• US 7,749,753 B2
• Filed: 04/09/2003
• Issued: 07/06/2010
• Est. Priority Date: 04/09/2002
• Status: Active Grant

• Current Assignee: Kyowa Kirin Co., Ltd. (Kirin Holdings Company Limited)
• Original Assignee: Kyowa Hakko Kirin Company Limited (Kirin Holdings Company Limited)
• Inventors: Mori, Katsuhiro, Satoh, Mitsuo, Kanda, Yutaka
• Primary Examiner(s): Chong; Kimberly
• Application Number:
  US10/409,600
• Publication Number:
  US 20040110282A1
• Time in Patent Office: 2,645 Days
• Field of Search: None
• US Class Current: 435/325
• CPC Class Codes: A61K 2039/505 comprising antibodies
• CPC Class Codes: A61P 29/00 Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
• CPC Class Codes: A61P 31/00 Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
• CPC Class Codes: A61P 31/04 Antibacterial agents
• CPC Class Codes: A61P 31/12 Antivirals
• CPC Class Codes: A61P 35/00 Antineoplastic agents
• CPC Class Codes: A61P 37/00 Drugs for immunological or allergic disorders
• CPC Class Codes: A61P 37/08 Antiallergic agents antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14
• CPC Class Codes: A61P 9/00 Drugs for disorders of the cardiovascular system
• CPC Class Codes: C07K 14/47 from mammals
• CPC Class Codes: C07K 16/18 against material from animals or humans
• CPC Class Codes: C07K 16/2866 against receptors for cytokines, lymphokines, interferons
• CPC Class Codes: C07K 2317/24 containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
• CPC Class Codes: C07K 2317/732 Antibody-dependent cellular cytotoxicity [ADCC]
• CPC Class Codes: C12N 15/8245 involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis

Abstract

A cell in which the activity of a protein relating to transport of an intracellular sugar nucleotide, GDP-fucose, to the Golgi body is more decreased or deleted than its parent cell; a process for producing an antibody composition using the cell; a transgenic non-human animal or plant or the progenies thereof, in which genome is modified so as to have a decreased or deleted activity of a protein relating to transport of an intracellular sugar nucleotide, GDP-fucose, to the Golgi body; a process for producing an antibody composition from the animal or plant; and a medicament comprising the antibody composition.

Claims

1. An isolated cell in which the activity of a GDP-fucose transporter is deleted by a gene disruption technique which comprises targeting a gene encoding a GDP-fucose transporter, wherein the gene encoding the GDP-fucose transporter is a DNA comprising the nucleotide sequence of SEQ ID NO:

1.

3. The cell according to claim 1 or claim 2, which is resistant to a lectin which recognizes a sugar chain in which 1-position of fucose is bound to 6-position of N-acetylglucosamine in the reducing end through α

-bond in a complex N-glycoside-linked sugar chain.

4. The cell according to claim 3, which is resistant to at least one lectin selected from the group consisting of the following (a) to (d):

(a) a Lens culinaris lectin;

(b) a Pisum sativum lectin;

(c) a Vicia faba lectin;

(d) an Aleuria aurantia lectin.

5. The cell according to claim 1 or claim 2, wherein the cell is an animal cell.

2. An isolated cell in which the activity of a GDP-fucose transporter is deleted by a gene disruption technique which comprises targeting a gene encoding a GDP-fucose transporter, wherein the GDP-fucose transporter is a protein comprising the amino acid sequence of SEQ ID NO:

2.

6. An isolated transformed cell in which the activity of a genomic GDP-fucose transporter is deleted, wherein the genomic GDP-fucose transporter is a protein comprising the amino acid sequence of SEQ ID NO:

2; and

wherein the activity of the genomic GDP-fucose transporter is deleted by an RNA interference (RNAi) method.

7. The cell according to claim 6, wherein the RNAi method uses a nucleotide represented by SEQ ID NO:

16.

8. The cell according to claim 6, which is resistant to a lectin which recognizes a sugar chain in which 1-position of fucose is bound to 6-position of N-acetylglucosamine in the reducing end through α

-bond in a complex N-glycoside-linked sugar chain.

9. The cell according to claim 8, which is resistant to at least one lectin selected from the group consisting of the following (a) to (d):

(a) a Lens culinaris lectin;

(b) a Pisum sativum lectin;

(c) a Vicia faba lectin;

(d) an Aleuria aurantia lectin.

10. The cell according to claim 6, wherein the cell is an animal cell.

11. The cell according to claim 10, wherein the animal cell is a CHO cell.