Methods and compositions for detection of microorganisms and cells and treatment of diseases and disorders
First Claim
1. A method for detecting a site of proliferation or a proliferative condition in a subject, comprising:
- administering an E. coli strain Nissle bacterium to a subject, wherein;
the subject has, is at risk of having or is suspected of having a proliferative condition; and
the Nissle bacterium expresses or accumulates a detectable molecule or a molecule that induces or produces a detectable signal; and
after a sufficient time for the Nissle bacterium to accumulate in proliferating tissues, detecting the detectable molecule or detectable signal to detect accumulation of Nissle bacterium in the subject, whereby detection of the Nissle bacterium indicates the site of proliferation or proliferative condition.
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Abstract
Described herein are methods for detecting a microorganism or cell in a subject and methods for detecting, imaging or diagnosing a site, disease, disorder or condition in a subject using microorganisms or cells. Also described are methods which use microorganisms or cells for treating a disease, disorder or condition. Such sites, diseases and disorders include sites of cell proliferation, proliferative conditions, neoplasms, tumors, neoplastic disease, wounds and inflammation. Further described are microorganisms and cells for use in the methods and compositions, combinations and kits, including diagnostic and pharmaceutical compositions, containing a microorganism or cell. Microorganisms and cells described herein include those that bind, sequester or accumulate metal, such as those that provide for metal acquisition, transport, storage and/or metabolism. Additional imaging and therapy agents are also described.
290 Citations
91 Claims
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1. A method for detecting a site of proliferation or a proliferative condition in a subject, comprising:
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administering an E. coli strain Nissle bacterium to a subject, wherein; the subject has, is at risk of having or is suspected of having a proliferative condition; and the Nissle bacterium expresses or accumulates a detectable molecule or a molecule that induces or produces a detectable signal; and after a sufficient time for the Nissle bacterium to accumulate in proliferating tissues, detecting the detectable molecule or detectable signal to detect accumulation of Nissle bacterium in the subject, whereby detection of the Nissle bacterium indicates the site of proliferation or proliferative condition. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91)
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Specification