Ocular property measuring apparatus and method therefor

• US 7,769,419 B2
• Filed: 10/20/2004
• Issued: 08/03/2010
• Est. Priority Date: 10/24/2003
• Status: Active Grant

• Current Assignee: Occuity Ltd
• Original Assignee: Lein Applied Diagnostics Limited
• Inventors: Daly, Daniel John
• Primary Examiner(s): Winakur; Eric F
• Assistant Examiner(s): Berhanu; Etsub D
• Application Number:
  US10/576,718
• Publication Number:
  US 20070078308A1
• Time in Patent Office: 2,113 Days
• Field of Search: 600/316, 600/318, 600/319
• US Class Current: 600/318
• CPC Class Codes: A61B 3/10 Objective types, i.e. instruments for examining the eyes independent of the patients' perceptions or reactions
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/6821 Eye

Abstract

A method and apparatus for measuring an apparent depth (1) of a section of an eye (30) are disclosed. Light is focused to a measurement location (15) proximate or within the eye. The measurement location is scanned through the section and upon passing through first and second refractive index interfaces defining the section, a respective reflected light signal is detected, from which apparent positions of the first and second interfaces may be derived. Preferably, a confocal scanning arrangement is employed. Preferably, the section is the aqueous humor (34) of the eye (30). From changes in its refractive index (n) corresponding changes in glucose concentration in the aqueous humor and, in turn, in the bloodstream of a patient may be derived, offering a non-invasive monitoring means for diabetic patients. The apparatus may be a hand-held device, employing microelectromechanical systems. The radius of curvature (R) of a curved section, such as a cornea (32) or ocular lens (36), may also be measured to determine refractive errors of the eye.

Claims

1. A method of measuring changes in an apparent depth of the anterior chamber of an eye, the anterior chamber being defined by a first interface between the cornea and the aqueous humor of the eye and a second interface between the aqueous humor and the ocular lens of the eye, the method comprising the steps of:

a) focusing light to a measurement location proximate or within the eye;

b) scanning the measurement location through the anterior chamber;

c) detecting reflected light from the measurement location as the measurement location passes through the first and the second interfaces and generating a signal representative of the detected light;

and using a processor;

d) deriving from the signal apparent positions of the first and the second interfaces and, therefrom, the apparent depth of the anterior chamber;

e) comparing the derived apparent depth with a previous reference measurement of the apparent depth, so as to determine a change in the refractive index of the aqueous humor; and

f) calculating a measure of change in concentration of an analyte of interest in the aqueous humor from the determined change of refractive index.

2. The method of claim 1, wherein the analyte of interest is glucose.

3. The method of claim 2, further comprising the step of calculating a measure of change in a concentration of glucose within the bloodstream of a patient.

4. The method of claim 1, wherein the analyte of interest is at least one of a naturally occurring or an intentionally introduced substance.

5. The method of claim 1, wherein the detected light is arranged to comprise substantially only light which has been focused to the measurement location and reflected by an interface of the eye.

6. The method of claim 1, wherein the scanning step (b) is achieved by one of translating a lens;

translating a lens and varying a numerical aperture (NA) of the lens;

translating a mirror of a mirror assembly;

varying a refractive index of a variable refractive index element;

or varying a focal length of a variable focal length lens.

7. The method of claim 1, wherein the signal peaks for points where the measurement location is coincident with an interface of the eye.

8. The method of claim 1, wherein the light has a single wavelength.

9. The method of claim 1, wherein the light comprises two or more wavelengths.

10. The method of claim 1, further comprising the prior step of providing a reference image, or object, to be focused by the eye during scanning, so as to enable the eye to be repeatably aligned.

11. An apparatus for measuring changes in an apparent depth of the anterior chamber of an eye, the anterior chamber being defined by a first interface between the cornea and the aqueous humor of the eye and a second interface between the aqueous humor and the ocular lens of the eye, the apparatus comprising:

a) an optical focusing assembly, adapted to focus incident light to a measurement location proximate or within the eye;

b) a scanning assembly, adapted to scan the measurement location through the anterior chamber;

c) a detector, adapted to detect reflected light from the measurement location as the measurement location passes through the first and the second interfaces and adapted to generate a signal representative of the detected light; and

d) a processor, adapted to;

i) derive from the signal apparent positions of the first and the second interfaces and, therefrom, the apparent depth of the anterior chamber;

ii) compare the derived apparent depth with a previous reference measurement of the apparent depth, so as to determine a change in the refractive index of the aqueous humor; and

iii) calculate a measure of change in concentration of an analyte of interest in the aqueous humor from the determined change of refractive index.

12. The apparatus of claim 11, the scanning assembly comprising a scanning stage, adapted to translate an element of the optical focussing assembly such that the measurement location is correspondingly scanned, wherein the processor is further adapted to track the translation of the element and thereby derive a position of the measurement location.

13. The apparatus of claim 11, wherein the detector is further arranged to detect substantially only light which has been focused to the measurement location and reflected by an interface of the eye.

14. The apparatus of claim 11, wherein the light has a single wavelength.

15. The apparatus of claim 11, wherein the light comprises two or more wavelengths.

16. The apparatus of claim 11, further comprising means to display a reference image, or object, for focusing by the eye during scanning, such that the eye may be repeatably aligned.

17. A micro-electromechanical system, comprising the apparatus of claim 11.

18. A hand-held device, comprising the micro-electromechanical system of claim 17.

19. A hand-held device, comprising the apparatus of claim 11.

20. A method of measuring changes in a property of an eye, comprising the steps of:

a) directing light from a light source to a first reference location;

b) spatially filtering light not received at the first reference location;

c) receiving light from the first reference location and focusing the light to a measurement location;

d) scanning the measurement location along a measurement line within the eye;

e) receiving reflected light from the measurement location and focusing the reflected light to a second reference location;

f) spatially filtering reflected light not received at the second reference location;

g) measuring an intensity of the reflected light received at the second reference location;

and using a processor;

h) relating an intensity measurement to an apparent position of the measurement location;

i) selecting intensity measurements of interest, the intensity measurements of interest representing measurement locations of interest; and

j) deriving a distance between the measurement locations of interest, the distance being an apparent depth of the anterior chamber, the anterior chamber being defined by a first interface between the cornea and the aqueous humor of the eye and a second interface between the aqueous humor and the ocular lens of the eye, the method further comprising the steps of;

k) comparing the derived apparent depth with a previous reference measurement of the apparent depth, so as to determine a change in the refractive index of the aqueous humor; and

l) calculating a measure of change in concentration of an analyte of interest in the aqueous humor from the determined change of refractive index.

21. The method of claim 20, wherein the first and second reference locations are coincident.

22. The method of claim 20, wherein scanning step (d) is achieved by one of translating a lens;

translating a lens and varying a numerical aperture (NA) of the lens;

translating a mirror of a mirror assembly;

varying a refractive index of a variable refractive index element;

or varying a focal length of a variable focal length lens.

23. The method of claim 20, further comprising controlling the light such that the light has one of a static, jittered, swept or stepped wavelength.

24. The method of claim 20, further comprising the steps of modulating the light and detecting the phase of the light received at the second reference location.

25. The method of claim 20, further comprising the step of generating light having two or more wavelengths, such that two or more properties of the eye may be measured.

26. The method of claim 20, further comprising the step of producing light having two or more polarization states, such that two or more properties of the eye may be measured.

27. The method of claim 20, further comprising the steps of:

i) producing a beam of coherent light;

ii) splitting the light beam into a probe beam and a reference beam, such that the probe beam is controlled according to the method of any one of claims 20 to 29;

iii) interfering the probe beam and the reference beam at a detector; and

iv) measuring a resulting interference pattern.

28. The method of claim 20, further comprising the step of effecting a reference accommodation of the eye by placing a reference object in a line of sight of the eye.

29. An apparatus for measuring changes in a property of an eye, the property being an apparent depth of the anterior chamber defined by a first interface between the cornea and the aqueous humor of the eye and a second interface between the aqueous humor and the ocular lens of the eye, the apparatus comprising:

a light source;

a source optical element, adapted to direct light from the light source to a first reference location;

an objective optical element, adapted to receive light from the first reference location and to focus the light to a measurement location, the objective optical element being further adapted to scan the measurement location along a measurement line within the eye and through the anterior chamber;

a return optical element, adapted to receive reflected light from the measurement location and to focus the reflected light to a second reference location;

an optical detector, adapted to measure an intensity of the reflected light received at the second reference location; and

a processor, adapted to;

i) relate intensity measurements of interest to apparent positions of the measurement location, so as to derive the apparent depth of the anterior chamber;

ii) compare the derived apparent depth with a previous reference measurement of the apparent depth, so as to determine a change in the refractive index of the aqueous humor; and

iii) calculate a measure of change in concentration of an analyte of interest in the aqueous humor from the determined change of refractive index.

30. The apparatus of claim 29, wherein the source optical element comprises one of a lens configuration, an optical fibre, or another light guide structure.

31. The apparatus of claim 29, wherein the first reference location is provided by one of a pinhole aperture, a source-detector combination, an optical fibre, or another light guide structure.

32. The apparatus of claim 29, wherein the objective optical element and/or the return optical element comprises a compound lens.

33. The apparatus of claim 32, further comprising a translation stage, adapted to translate a lens of the compound lens and thereby to scan the measurement location along the measurement line.

34. The apparatus of claim 29, wherein the objective optical element and the return optical element are constituted by the same optical element.

35. The apparatus of claim 34, wherein the first and second reference locations are coincident.

36. The apparatus of claim 29, wherein the light source comprises a white light source and one of a spectrometer, an etalon, or a multiplexer.

37. The apparatus of claim 29, further comprising a reference object for viewing by the eye, the reference object being positioned such that an accommodation of the eye may be repeatably achieved.

38. A micro-electromechanical system, comprising the apparatus of claim 29.

39. A hand-held device, comprising the micro-electromechanical system of claim 38.

40. A hand-held device, comprising the apparatus of claim 29.