Method and kits for detecting cerebrospinal fluid in a sample

• US 7,794,953 B2
• Filed: 04/26/2007
• Issued: 09/14/2010
• Est. Priority Date: 04/09/2003
• Status: Active Grant

• Current Assignee: Stony Brook Anaesthesiology, University Faculty Practice Corporation
• Original Assignee: Stony Brook Anaesthesiology, University Faculty Practice Corporation
• Inventors: Pentyala, Srinivas N.
• Primary Examiner(s): Shibuya; Mark L
• Assistant Examiner(s): FOSTER, CHRISTINE E
• Application Number:
  US11/789,868
• Publication Number:
  US 20070196864A1
• Time in Patent Office: 1,237 Days
• Field of Search: None
• US Class Current: 435/7.1
• CPC Class Codes: C12Q 1/527 involving lyase
• CPC Class Codes: G01N 2333/99 Isomerases (5.)
• CPC Class Codes: G01N 33/573 for enzymes or isoenzymes
• CPC Class Codes: G01N 33/6896 Neurological disorders, e.g. Alzheimer's disease

Abstract

The present invention relates to detection of the presence or absence of cerebrospinal fluid (CSF) in a sample, in particular to the analysis of the CSF protein lipocalin-type prostaglandin D2 synthase (L-PGDS). The present invention provides assays for the analysis of PGDS indicating the presence or absence of CSF in a sample.

Claims

1. A method for detecting the presence or absence of cerebrospinal fluid in a fluid sample from a needle placed in the epidural space to administer epidural medication or a fluid sample from a catheter attached to the needle, comprising

analyzing the fluid sample from a needle placed in the epidural space to administer epidural medication or the fluid sample from a catheter attached to the needle for binding with an antibody against lipocalin-type prostaglandin D2 synthase;

and

correlating the presence or absence of binding with the antibody with the presence or absence of cerebrospinal fluid in the sample.

2. The method of claim 1, wherein the antibody is an antibody generated against a lipocalin-type prostaglandin D2 synthase peptide epitope.