Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminoglycanases

CAFC
  • US 7,829,081 B2
  • Filed: 03/04/2010
  • Issued: 11/09/2010
  • Est. Priority Date: 02/23/2005
  • Status: Active Grant
First Claim
Patent Images

1. A pharmaceutical composition, comprising:

  • a) a hyaluronidase glycoprotein that is active at neutral pH and contains at least one sugar moiety that is covalently attached to an asparagine (N) residue of the hyaluronidase polypeptide, wherein;

    (i) the hyaluronidase glycoprotein consists of the sequence of amino acids set forth as amino acids 36-477, 36-478, 36-479, 36-480, 36-481, 36-482, or 36-483 of SEQ ID NO;

    1;

    or(ii) the hyaluronidase glycoprotein contains amino acid substitutions in the sequence of amino acids set forth as amino acids 36-477, 36-478, 36-479, 36-480, 36-481, 36-482, or 36-483 of SEQ ID NO;

    1, whereby the amino acid-substituted hyaluronidase glycoprotein consists of a sequence of amino acids that has at least 95% amino acid sequence identity with the sequence of amino acids set forth as amino acids 36-477, 36-478, 36-479, 36-480, 36-481, 36-482, or 36-483 of SEQ ID NO;

    1; and

    b) a cosmetic agent.

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